Electrotaxis of cardiac progenitor cells, cardiac fibroblasts, and induced pluripotent stem cell-derived cardiac progenitor cells requires serum and is directed via PI3′K pathways

Content Provider	Scilit
Author	Frederich, Bert J. Timofeyev, Valeriy Thai, Phung N. Haddad, Michael J. Poe, Adam J. Lau, Victor C. Moshref, Maryam Knowlton, Anne A. Sirish, Padmini Chiamvimonvat, Nipavan
Copyright Year	2017
Description	Journal: Heart rhythm Background The limited regenerative capacity of cardiac tissue has long been an obstacle to treating damaged myocardium. Cell-based therapy offers an enormous potential to the current treatment paradigms. However, the efficacy of regenerative therapies remains limited by inefficient delivery and engraftment. Electrotaxis (electrically guided cell movement) has been clinically used to improve recovery in a number of tissues but has not been investigated for treating myocardial damage. Objective The purpose of this study was to test the electrotactic behaviors of several types of cardiac cells. Methods Cardiac progenitor cells (CPCs), cardiac fibroblasts (CFs), and human induced pluripotent stem cell-derived cardiac progenitor cells (hiPSC-CPCs) were used. Results CPCs and CFs electrotax toward the anode of a direct current electric field, whereas hiPSC-CPCs electrotax toward the cathode. The voltage-dependent electrotaxis of CPCs and CFs requires the presence of serum in the media. Addition of soluble vascular cell adhesion molecule to serum-free media restores directed migration. We provide evidence that CPC and CF electrotaxis is mediated through phosphatidylinositide 3-kinase signaling. In addition, very late antigen-4, an integrin and growth factor receptor, is required for electrotaxis and localizes to the anodal edge of CPCs in response to direct current electric field. The hiPSC-derived CPCs do not express very late antigen-4, migrate toward the cathode in a voltage-dependent manner, and, similar to CPCs and CFs, require media serum and phosphatidylinositide 3-kinase activity for electrotaxis. Conclusion The electrotactic behaviors of these therapeutic cardiac cells may be used to improve cell-based therapy for recovering function in damaged myocardium.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671891/pdf http://www.heartrhythmjournal.com/article/S1547527117308408/pdf
Ending Page	1692
Page Count	8
Starting Page	1685
ISSN	15475271
e-ISSN	15563871
DOI	10.1016/j.hrthm.2017.06.038
Journal	Heart rhythm
Issue Number	11
Volume Number	14
Language	English
Publisher	Elsevier BV
Publisher Date	2017-06-28
Access Restriction	Open
Subject Keyword	Journal: Heart rhythm Cardiology and Cardiovascular Diseases Cardiac Fibroblast Cardiac Progenitor Cells Human Induced Pluripotent Stem Cells Phosphatidylinositide-3-kinase Soluble Vascular Cell Adhesion Molecule Cardiac Progenitor Cell Human Induced Pluripotent Stem Cell-derived Cardiac Progenitor Cell Quantitative Polymerase Chain Reaction Small Interfering Ribonucleic Acid
Content Type	Text
Resource Type	Article
Subject	Physiology (medical) Cardiology and Cardiovascular Medicine