Nanoindentation modulus of murine cartilage: a sensitive indicator of the initiation and progression of post-traumatic osteoarthritis

Content Provider	Scilit
Author	Doyran, B. Tong, W. Li, Q. Jia, H. Zhang, X. Chen, C. Enomoto-Iwamoto, M. Lu, X. L. Qin, L. Han, L.
Copyright Year	2016
Description	Journal: Osteoarthritis and cartilage Summary Objective This study aims to demonstrate that cartilage nanoindentation modulus is a highly sensitive indicator of the onset and spatiotemporal progression of post-traumatic osteoarthritis (PTOA) in murine models. Design Destabilization of the medial meniscus (DMM) surgery was performed on the right knees of 12-week old male, wild-type C57BL/6 mice, with Sham control on contralateral left knees. Atomic force microscopy (AFM)-based nanoindentation was applied to quantify the nanoindentation modulus, $E_{ind}$, of femoral condyle cartilage at 3 days to 12 weeks after surgery. The modulus changes were compared against the timeline of histological OA signs. Meanwhile, at 8 weeks after surgery, changes in meniscus, synovium and subchondral bone were evaluated to reveal the spatial progression of PTOA. Results The modulus of medial condyle cartilage was significantly reduced at 1 week after DMM, preceding the histological OA signs, which only became detectable at 4–8 weeks after. This reduction is likely due to concomitantly elevated proteolytic activities, as blocking enzymatic activities in mice can attenuate this modulus reduction. In later OA, lateral condyle cartilage and medial meniscus also started to be weakened, illustrating the whole-organ nature of PTOA. Conclusions This study underscores the high sensitivity of nanoindentation in examining the initiation, attenuation and progression of PTOA in murine models. Meanwhile, modulus changes highlight concomitant changes in lateral cartilage and meniscus during the advancement of OA.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5182132/pdf http://www.oarsijournal.com/article/S1063458416302436/pdf
Ending Page	117
Page Count	10
Starting Page	108
ISSN	10634584
e-ISSN	15229653
DOI	10.1016/j.joca.2016.08.008
Journal	Osteoarthritis and cartilage
Issue Number	1
Volume Number	25
Language	English
Publisher	Elsevier BV
Publisher Date	2016-08-25
Access Restriction	Open
Subject Keyword	Journal: Osteoarthritis and cartilage Post-traumatic Osteoarthritis Destabilization of the Medial Meniscus (dmm)
Content Type	Text
Resource Type	Article
Subject	Orthopedics and Sports Medicine Biomedical Engineering Rheumatology