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Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models
| Content Provider | Scilit |
|---|---|
| Author | Bottini, Massimo Mebarek, Saida Anderson, Karen L. Strzelecka-Kiliszek, Agnieszka Bozycki, Lukasz Simão, Ana Maria Sper Bolean, Maytê Ciancaglini, Pietro Pikula, Joanna Bandorowicz Pikula, Slawomir Magne, David Volkmann, Niels Hanein, Dorit Millán, José Luis Buchet, Rene |
| Copyright Year | 2018 |
| Description | Journal: Biochimica Et Biophysica Acta (bba) - General Subjects Matrix vesicles (MVs) are released from hypertrophic chondrocytes and from mature osteoblasts, the cells responsible for endochondral and membranous ossification. Under pathological conditions, they can also be released from cells of non-skeletal tissues such as vascular smooth muscle cells. MVs are extracellular vesicles of approximately 100-300nm diameter harboring the biochemical machinery needed to induce mineralization. The review comprehensively delineates our current knowledge of MV biology and highlights open questions aiming to stimulate further research. The review is constructed as a series of questions addressing issues of MVs ranging from their biogenesis and functions, to biomimetic models. It critically evaluates experimental data including their isolation and characterization methods, like lipidomics, proteomics, transmission electron microscopy, atomic force microscopy and proteoliposome models mimicking MVs. MVs have a relatively well-defined function as initiators of mineralization. They bind to collagen and their composition reflects the composition of lipid rafts. We call attention to the as yet unclear mechanisms leading to the biogenesis of MVs, and how minerals form and when they are formed. We discuss the prospects of employing upcoming experimental models to deepen our understanding of MV-mediated mineralization and mineralization disorders such as the use of reconstituted lipid vesicles, proteoliposomes and, native sample preparations and high-resolution technologies. MVs have been extensively investigated owing to their roles in skeletal and ectopic mineralization. MVs serve as a model system for lipid raft structures, and for the mechanisms of genesis and release of extracellular vesicles. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801150/pdf |
| Ending Page | 546 |
| Page Count | 15 |
| Starting Page | 532 |
| ISSN | 03044165 |
| DOI | 10.1016/j.bbagen.2017.11.005 |
| Journal | Biochimica Et Biophysica Acta (bba) - General Subjects |
| Issue Number | 3 |
| Volume Number | 1862 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2018-03-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Biochimica Et Biophysica Acta (bba) - General Subjects Atomic Force Microscopy Electron Microscopy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Biochemistry Biophysics |
