Chemical and Biophysical Approaches for Complete Characterization of Lectin–Carbohydrate Interactions

Content Provider	Scilit
Author	Lusvarghi, Sabrina Ghirlando, Rodolfo Davison, Jack R. Bewley, Carole A.
Copyright Year	2017
Description	Lectins are carbohydrate-binding proteins unrelated to antibodies or enzymes. While carbohydrates are present on all cells and pathogens, lectins are also ubiquitous in nature and their interactions with glycans mediate countless biological and physical interactions. Due to the multivalency found in both lectins and their glycan-binding partners, complete characterization of these interactions can be complex and typically requires the use of multiple complimentary techniques. In this chapter, we provide a general strategy and protocols for chemical and biophysical approaches that can be used to characterize carbohydrate-mediated interactions in the context of individual oligosaccharides, as part of a glycoprotein, and ending with visualization of interactions with whole virions. Book Name: Protein \3- DNA Interactions
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141027/pdf
DOI	10.1016/bs.mie.2017.06.010
Volume Number	598
Language	English
Publisher	Elsevier BV
Publisher Date	2017-07-23
Access Restriction	Open
Subject Keyword	Book Name: Protein \3- DNA Interactions Physical Chemistry Surface Plasmon Resonance Electron Microscopy Analytical Ultracentrifugation Dynamic Light Scattering High-mannose Oligosaccharides
Content Type	Text
Resource Type	Article