Large scale preparation of high mannose and paucimannose N-glycans from soybean proteins by oxidative release of natural glycans (ORNG)

Content Provider	Scilit
Author	Zhu, Yuyang Yan, Maomao Lasanajak, Yi Smith, David F. Song, Xuezheng
Copyright Year	2018
Description	Journal: Carbohydrate research Despite the important advances in chemical and chemoenzymatic synthesis of glycans, access to large quantities of complex natural glycans remains a major impediment to progress in Glycoscience. Here we report a large-scale preparation of N-glycans from a kilogram of commercial soy proteins using oxidative release of natural glycans (ORNG). The high mannose and paucimannose N-glycans were labeled with a fluorescent tag and purified by size exclusion and multidimensional preparative HPLC. Side products are identified and potential mechanisms for the oxidative release of natural N-glycans from glycoproteins are proposed. This study demonstrates the potential for using the ORNG approach as a complementary route to synthetic approaches for the preparation of multi-milligram quantities of biomedically relevant complex glycans.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6309449/pdf
Ending Page	27
Page Count	9
Starting Page	19
ISSN	00086215
e-ISSN	1873426X
DOI	10.1016/j.carres.2018.05.002
Journal	Carbohydrate research
Volume Number	464
Language	English
Publisher	Elsevier BV
Publisher Date	2018-05-15
Access Restriction	Open
Subject Keyword	Journal: Carbohydrate research Analytical Chemistry Oxidative Release Natural Glycans Sodium Hypochlorite
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Medicine Analytical Chemistry Biochemistry