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Regulation of yeast fatty acid desaturase in response to iron deficiency
| Content Provider | Scilit |
|---|---|
| Author | Romero, Antonia María Jordá, Tania Rozès, Nicolas Martínez-Pastor, María Teresa Puig, Sergi |
| Copyright Year | 2018 |
| Description | Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Unsaturated fatty acids (UFA) are essential components of phospholipids that greatly contribute to the biophysical properties of cellular membranes. Biosynthesis of UFAs relies on a conserved family of iron-dependent fatty acid desaturases, whose representative in the model yeast Saccharomyces cerevisiae is Ole1. OLE1 expression is tightly regulated to adapt UFA biosynthesis and lipid bilayer properties to changes in temperature, and in UFA or oxygen availability. Despite iron deficiency being the most extended nutritional disorder worldwide, very little is known about the mechanisms and the biological relevance of fatty acid desaturases regulation in response to iron starvation. In this report, we show that endoplasmic reticulum-anchored transcription factor Mga2 activates OLE1 transcription in response to nutritional and genetic iron deficiencies. Cells lacking MGA2 display low UFA levels and do not grow under iron-limited conditions, unless UFAs are supplemented or OLE1 is overexpressed. The proteasome, E3 ubiquitin ligase Rsp5 and the $Cdc48^{Npl4/Ufd1}$ complex are required for OLE1 activation during iron depletion. Interestingly, Mga2 also activates the transcription of its own mRNA in response to iron deficiency, hypoxia, low temperature and low UFAs. MGA2 up-regulation contributes to increase OLE1 expression in these situations. These results reveal the mechanism of OLE1 regulation when iron is scarce and identify the MGA2 auto-regulation as a potential activation strategy in multiple stresses. |
| Related Links | https://digital.csic.es/bitstream/10261/164001/1/BBA-2018-Romero.pdf |
| Ending Page | 668 |
| Page Count | 12 |
| Starting Page | 657 |
| ISSN | 13881981 |
| e-ISSN | 18792618 |
| DOI | 10.1016/j.bbalip.2018.03.008 |
| Journal | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids |
| Issue Number | 6 |
| Volume Number | 1863 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2018-04-05 |
| Access Restriction | Open |
| Subject Keyword | Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Biochemistry and Molecular Biology Cell Biology Saccharomyces Cerevisiae Iron Deficiency Fatty Acids |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology |
