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c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium
| Content Provider | Scilit |
|---|---|
| Author | Sehgal, Anuj Kobayashi, Atsushi Donaldson, David S. Mabbott, Neil A. |
| Copyright Year | 2017 |
| Description | Journal: Immunobiology M cells reside within the follicle-associated epithelium (FAE) overlying the gut-associated lymphoid tissues. These unique phagocytic epithelial cells enable the mucosal immune system to sample antigens within the lumen of the intestine. The differentiation of M cells from uncommitted precursors in the FAE is dependent on the production of receptor activator of nuclear factor-κB ligand (RANKL) by subepithelial stromal cells. The ligation of a variety of cell surface receptors activates the nuclear factor-κB (NF-κB) family of transcription factors which in-turn induce the transcription of multiple target genes. RANKL-stimulation can stimulate the nuclear translocation of the NF-κB subunit c-Rel. We therefore used c-Rel-deficient mice to determine whether the differentiation and functional maturation of M cells in the Peyer’s patches was dependent on c-Rel. Our data show that c-Rel-deficiency does not influence the expression of RANKL or RANK in Peyer’s patches, or the induction of M-cell differentiation in the FAE. RANKL-stimulation in the differentiating M cells induces the expression of SpiB which is essential for their subsequent maturation. However, SpiB expression in the FAE was also unaffected in the absence of c-Rel. As a consequence, the functional maturation of M cells was not impaired in the Peyer’s patches of c-Rel-deficient mice. Although our data showed that the specific expression of CCL20 and ubiquitin D in the FAE was not impeded in the absence of c-Rel, the expression of ubiquitin D was dramatically reduced in the B cell-follicles of c-Rel-deficient mice. Coincident with this, we also observed that the status of follicular dendritic cells in the B cell-follicles was dramatically reduced in Peyer’s patches from c-Rel-deficient mice. Taken together, our data show that c-Rel is dispensable for the RANKL-mediated differentiation and functional maturation of M cells. |
| Related Links | https://core.ac.uk/download/pdf/82598577.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5152706/pdf |
| Ending Page | 326 |
| Page Count | 11 |
| Starting Page | 316 |
| ISSN | 01712985 |
| DOI | 10.1016/j.imbio.2016.09.008 |
| Journal | Immunobiology |
| Issue Number | 2 |
| Volume Number | 222 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2017-02-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Immunobiology Anxa5, Annexin A5 Fae, Follicle-associated Epithelium Fdc, Follicular Dendritic Cell Galt, Gut-associated Lymphoid Tissues Ihc, Immunohistochemistry Marcksl1, Myristoylated Alanine-rich C-kinase Substrate-like Protein 1 Nf-κb, Nuclear Factor-κb Rank, Receptor Activator of Nuclear Factor-κb Rankl, Receptor Activator of Nuclear Factor-κb Ligand Scg5, Secretogranin V Sed, Sub-epithelial Dome Uea-1, Ulex Europaeus Agglutinin-1 Follicle-associated Epithelium |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Hematology Immunology |
