NDLI: One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays

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One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays

Content Provider	Scilit
Author	Dai, Sheng Li, Rong Long, Yan Titus, Steve Zhao, Jinghua Huang, Ruili Xia, Menghang Zheng, Wei
Copyright Year	2016
Description	Journal: SLAS Discovery Human neuronal cells differentiated from induced pluripotent cells have emerged as a new model system for the study of disease pathophysiology and evaluation of drug efficacy. Differentiated neuronal cells are more similar in genetics and biological content to human brain cells than other animal disease models. However, culture of neuronal cells in assay plates requires a labor-intensive procedure of plate precoating, hampering its applications in high-throughput screening (HTS). We developed a simplified method with one-step seeding of neural stem cells in assay plates by supplementing the medium with a recombinant human vitronectin (VTN), thus avoiding plate precoating. Robust results were obtained from cell viability, calcium response, and neurite outgrowth assays using this new method. Our data demonstrate that this approach greatly simplifies high-throughput assays using neuronal cells differentiated from human stem cells for translational research.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5285267/pdf https://journals.sagepub.com/doi/pdf/10.1177/1087057116670068
Ending Page	1124
Page Count	13
Starting Page	1112
ISSN	24725552
e-ISSN	24725560
DOI	10.1177/1087057116670068
Journal	SLAS Discovery
Issue Number	10
Volume Number	21
Language	English
Publisher	Elsevier BV
Publisher Date	2016-09-19
Access Restriction	Open
Subject Keyword	Journal: SLAS Discovery Cell Tissue Engineering High-content Screening High-throughput Screening Induced Pluripotent Stem Cells Neural Stem Cells Neuronal Cells Plate Coating
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Biochemistry Molecular Medicine Biotechnology

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