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A novel platelet lysate hydrogel for endothelial cell and mesenchymal stem cell-directed neovascularization
| Content Provider | Scilit |
|---|---|
| Author | Robinson, Scott T. Douglas, Alison M. Chadid, Tatiana Kuo, Katie Rajabalan, Ajai Li, Haiyan Copland, Ian B. Barker, Thomas H. Galipeau, Jacques Brewster, Luke P. |
| Copyright Year | 2016 |
| Description | Journal: Acta biomaterialia Mesenchymal stem cells (MSC) hold promise in promoting vascular regeneration of ischemic tissue in conditions like critical limb ischemia of the leg. However, this approach has been limited in part by poor cell retention and survival after delivery. New biomaterials offer an opportunity to localize cells to the desired tissue after delivery, but also to improve cell survival after delivery. Here we characterize the mechanical and microstructural properties of a novel hydrogel composed of pooled human platelet lysate (PL) and test its ability to promote MSC angiogenic activity using clinically relevant in vitro and in vivo models. This PL hydrogel had comparable storage and loss modulus and behaved as a viscoelastic solid similar to fibrin hydrogels despite having 1/4–1/10th the fibrin content of standard fibrin gels. Additionally, PL hydrogels enabled sustained release of endogenous PDGF-BB for up to 20 days and were resistant to protease degradation. PL hydrogel stimulated pro-angiogenic activity by promoting human MSC growth and invasion in a 3D environment, and enhancing endothelial cell sprouting alone and in co-culture with MSCs. When delivered in vivo, the combination of PL and human MSCs improved local tissue perfusion after 8 days compared to controls when assessed with laser Doppler perfusion imaging in a murine model of hind limb ischemia. These results support the use of a PL hydrogel as a scaffold for MSC delivery to promote vascular regeneration. |
| Related Links | http://europepmc.org/articles/pmc4846562?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846562/pdf |
| Ending Page | 98 |
| Page Count | 13 |
| Starting Page | 86 |
| ISSN | 17427061 |
| e-ISSN | 18787568 |
| DOI | 10.1016/j.actbio.2016.03.002 |
| Journal | Acta biomaterialia |
| Volume Number | 36 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2016-03-04 |
| Access Restriction | Open |
| Subject Keyword | Journal: Acta biomaterialia Mesenchymal Stem Cell |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biochemistry Molecular Biology Biomaterials Biomedical Engineering Biotechnology |
