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Steps for Shigella Gatekeeper Protein MxiC Function in Hierarchical Type III Secretion Regulation
| Content Provider | Scilit |
|---|---|
| Author | Roehrich, A. Dorothea Bordignon, Enrica Mode, Selma Shen, Da-Kang Liu, Xia Pain, Maria Murillo, Isabel Martinez-Argudo, Isabel Sessions, Richard B. Blocker, Ariel J. |
| Copyright Year | 2017 |
| Description | Journal: Journal of Biological Chemistry Type III secretion systems are complex nanomachines used for injection of proteins from Gram-negative bacteria into eukaryotic cells. Although they are assembled when the environmental conditions are appropriate, they only start secreting upon contact with a host cell. Secretion is hierarchical. First, the pore-forming translocators are released. Second, effector proteins are injected. Hierarchy between these protein classes is mediated by a conserved gatekeeper protein, MxiC, in Shigella. As its molecular mechanism of action is still poorly understood, we used its structure to guide site-directed mutagenesis and to dissect its function. We identified mutants predominantly affecting all known features of MxiC regulation as follows: secretion of translocators, MxiC and/or effectors. Using molecular genetics, we then mapped at which point in the regulatory cascade the mutants were affected. Analysis of some of these mutants led us to a set of electron paramagnetic resonance experiments that provide evidence that MxiC interacts directly with IpaD. We suggest how this interaction regulates a switch in its conformation that is key to its functions. |
| Related Links | http://www.jbc.org/content/292/5/1705.full.pdf https://core.ac.uk/download/pdf/77602881.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5290946/pdf http://www.jbc.org/article/S0021925820367016/pdf |
| Ending Page | 1723 |
| Page Count | 19 |
| Starting Page | 1705 |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| DOI | 10.1074/jbc.m116.746826 |
| Journal | Journal of Biological Chemistry |
| Issue Number | 5 |
| Volume Number | 292 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2017-02-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Chemistry Cell Biology Cellular Regulation Electron Paramagnetic Resonance (epr) Gram-negative Bacteria Molecular Genetics Protein Secretion Type Iii Secretion System (t3ss) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
