NDLI: Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach

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Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach

Content Provider	Scilit
Author	Neumann-Schaal, Meina Metzendorf, Nicole G. Troitzsch, Daniel Nuss, Aaron Mischa Hofmann, Julia Danielle Beckstette, Michael Dersch, Petra Otto, Andreas Sievers, Susanne
Copyright Year	2018
Description	Journal: Anaerobe Clostridioides difficile is the major pathogen causing diarrhea following antibiotic treatment. It is considered to be a strictly anaerobic bacterium, however, previous studies have shown a certain and strain-dependent oxygen tolerance. In this study, the model strain C. difficile 630Δerm was shifted to micro-aerobiosis and was found to stay growing to the same extent as anaerobically growing cells with only few changes in the metabolite pattern. However, an extensive change in gene expression was determined by RNA-Seq. The most striking adaptation strategies involve a change in the reductive fermentation pathways of the amino acids proline, glycine and leucine. But also a far-reaching restructuring in the carbohydrate metabolism was detected with changes in the phosphotransferase system (PTS) facilitated uptake of sugars and a repression of enzymes of glycolysis and butyrate fermentation. Furthermore, a temporary induction in the synthesis of cofactor riboflavin was detected possibly due to an increased demand for flavin mononucleotid (FMN) and flavin adenine dinucleotide (FAD) in redox reactions. However, biosynthesis of the cofactors thiamin pyrophosphate and cobalamin were repressed deducing oxidation-prone enzymes and intermediates in these pathways.
Related Links	https://repository.helmholtz-hzi.de/bitstream/10033/621411/1/Neumann-Schaal_et_al.pdf
ISSN	10759964
e-ISSN	10958274
DOI	10.1016/j.anaerobe.2018.05.018
Journal	Anaerobe
Volume Number	53
Language	English
Publisher	Elsevier BV
Publisher Date	2018-05-31
Access Restriction	Open
Subject Keyword	Journal: Anaerobe Biochemistry and Molecular Biology Clostridioides Difficile Cysteine Modification
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology

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