Preparation and Biochemical Analysis of Classical Histone Deacetylases

Content Provider	Scilit
Author	Villagra, A. Sahakian, E. Seto, E.
Copyright Year	2016
Description	Histone deacetylase assays were first developed in the 1970s, and subsequently refined in the 1990s with the cloning of HDAC enzymes. Most of these early assays, relying on traditional in vitro chemical methodologies, are still applicable today. More recently, however, cell-based HDAC assays that measure HDAC activities in physiological conditions are emerging. Also, there is a continuing development of assays that can measure an isolated HDAC in the absence of other HDAC activities. This chapter reviews some of the older established methods for assaying HDAC activities, as well as introduces more recently developed nontraditional assays. Book Name: Protein \3- DNA Interactions
Related Links	http://europepmc.org/articles/pmc5557005?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557005/pdf
Ending Page	181
Page Count	21
Starting Page	161
DOI	10.1016/bs.mie.2016.04.005
Volume Number	573
Language	English
Publisher	Elsevier BV
Publisher Date	2016-05-31
Access Restriction	Open
Subject Keyword	Book Name: Protein \3- DNA Interactions Histone Deacetylase Hdac Assay Hdac Inhibitor Hdac Substrate
Content Type	Text
Resource Type	Article