Loading...
Please wait, while we are loading the content...
Similar Documents
Development of a neuroprotective potential algorithm for medicinal plants
| Content Provider | Scilit |
|---|---|
| Author | Liu, Weixi Ma, Hang DaSilva, Nicholas A. Rose, Kenneth N. Johnson, Shelby L. Zhang, Lu Wan, Chunpeng Dain, Joel A. Seeram, Navindra P. |
| Copyright Year | 2016 |
| Description | Journal: Neurochemistry international Medicinal plants are promising candidates for Alzheimer's disease (AD) research but there is lack of systematic algorithms and procedures to guide their selection and evaluation. Herein, we developed a Neuroprotective Potential Algorithm (NPA) by evaluating twenty-three standardized and chemically characterized Ayurvedic medicinal plant extracts in a panel of bioassays targeting oxidative stress, carbonyl stress, protein glycation, amyloid beta (Aβ) fibrillation, acetylcholinesterase (AChE) inhibition, and neuroinflammation. The twenty-three herbal extracts were initially evaluated for: 1) total polyphenol content (Folin-Ciocalteu assay), 2) free radical scavenging capacity (DPPH assay), 3) ferric reducing antioxidant power (FRAP assay), 4) reactive carbonyl species scavenging capacity (methylglyoxal trapping assay), 5) anti-glycative effects (BSA-fructose, and BSA-methylglyoxal assays) and, 6) anti-Aβ fibrillation effects (thioflavin-T assay). Based on assigned index scores from the initial screening, twelve extracts with a cumulative NPA score ≥40 were selected for further evaluation for their: 1) inhibitory effects on AChE activity, 2) in vitro anti-inflammatory effects on murine BV-2 microglial cells (Griess assay measuring levels of lipopolysaccharide-induced nitric oxide species), and 3) in vivo neuroprotective effects on Caenorhabditis elegans post induction of Aβ1-42 induced neurotoxicity and paralysis. Among these, four extracts had a cumulative NPA score ≥60 including Phyllanthus emblica (amla; Indian gooseberry), Mucuna pruriens (velvet bean), Punica granatum (pomegranate) and Curcuma longa (turmeric; curcumin). These extracts also showed protective effects on H2O2 induced cytotoxicity in differentiated cholinergic human neuronal SH-SY5Y and murine BV-2 microglial cells and reduced tau protein levels in the SH-SY5Y neuronal cells. While published animal data support the neuroprotective effects of several of these Ayurvedic medicinal plant extracts, some remain unexplored for their anti-AD potential. Therefore, the NPA may be utilized, in part, as a strategy to help guide the selection of promising medicinal plant candidates for future AD-based research using animal models. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191989/pdf |
| Ending Page | 177 |
| Page Count | 14 |
| Starting Page | 164 |
| ISSN | 01970186 |
| e-ISSN | 18729754 |
| DOI | 10.1016/j.neuint.2016.09.014 |
| Journal | Neurochemistry international |
| Volume Number | 100 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2016-11-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Neurochemistry international Integrative and Complementary Medicine Alzheimer's Disease (ad) Beta Amyloid (aβ) Caenorhabditis Elegans Neuroinflammation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Cellular and Molecular Neuroscience |
