NDLI: Cobalt-based paramagnetic probe to study RNA-protein interactions by NMR

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Cobalt-based paramagnetic probe to study RNA-protein interactions by NMR

Content Provider	Scilit
Author	Seebald, Leah M. DeMott, Christopher M. Ranganathan, Srivathsan Asare-Okai, Papa Nii Glazunova, Anastasia Chen, Alan Shekhtman, Alexander Royzen, Maksim
Copyright Year	2017
Description	Journal: Journal of inorganic biochemistry Paramagnetic resonance enhancement (PRE) is an NMR technique that allows studying three-dimensional structures of RNA-protein complexes in solution. RNA strands are typically spin labeled using nitroxide reagents, which provide minimal perturbation to the native structure. The current work describes an alternative approach, which is based on a Co2+-based probe that can be covalently attached to RNA in the vicinity of the protein's binding site using 'click' chemistry. Similar to nitroxide spin labels, the transition metal based probe is capable of attenuating NMR signal intensities from protein residues localized <40Å away. The extent of attenuation is related to the probe's distance, thus allowing for construction of the protein's contact surface map. This new paradigm has been applied to study binding of HIV-1 nucleocapsid protein 7, NCp7, to a model RNA pentanucleotide.
Related Links	http://europepmc.org/articles/pmc5956527?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5956527/pdf
Ending Page	208
Page Count	7
Starting Page	202
ISSN	01620134
e-ISSN	18733344
DOI	10.1016/j.jinorgbio.2017.02.024
Journal	Journal of inorganic biochemistry
Volume Number	170
Language	English
Publisher	Elsevier BV
Publisher Date	2017-05-01
Access Restriction	Open
Subject Keyword	Journal: Journal of inorganic biochemistry Analytical Chemistry Rna-protein Interactions
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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