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The isolation and characterization of a new snake venom cysteine-rich secretory protein (svCRiSP) from the venom of the Southern Pacific rattlesnake and its effect on vascular permeability
| Content Provider | Scilit |
|---|---|
| Author | Suntravat, Montamas Cromer, Walter E. Marquez, Jessenia Galan, Jacob A. Zawieja, David C. Davies, Peter Salazar, Emelyn Sánchez, Elda E. |
| Copyright Year | 2019 |
| Description | Journal: Toxicon A novel snake venom cysteine-rich secretory protein (svCRiSP), Hellerin, was purified from C. o. helleri venom using sequential reverse phase and cation-exchange chromatography. Gel electrophoresis, N-terminal sequencing, and LC-MS/MS sequencing identified a single protein with a molecular mass of approximately 24.8 kDa and confirmed its identity as a svCRiSP. Hellerin had cytotoxic effects on human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner but not in human dermal lymphatic endothelial cells (HDLECs) and human dermal blood endothelial cells (HDBECs). Hellerin produced a dramatic increase in both blood vascular permeability in vivo, and in the trans-epithelial permeability of cultured HDLEC and HDBEC cells. This is the first study that describes the effect of a svCRiSP on vascular, blood and lymphatic permeability. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556137/pdf |
| Ending Page | 30 |
| Page Count | 9 |
| Starting Page | 22 |
| ISSN | 00410101 |
| e-ISSN | 18793150 |
| DOI | 10.1016/j.toxicon.2019.04.006 |
| Journal | Toxicon |
| Volume Number | 165 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2019-04-20 |
| Access Restriction | Open |
| Subject Keyword | Journal: Toxicon Snake Venom Southern Pacific Rattlesnake Snake Venom Cysteine-rich Secretory Protein (svcrisp) Vascular Permeability Lymphatic Cells |
| Content Type | Text |
| Resource Type | Article |
| Subject | Toxicology |
