NDLI: An Anatomically Resolved Mouse Brain Proteome Reveals Parkinson Disease-relevant Pathways

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An Anatomically Resolved Mouse Brain Proteome Reveals Parkinson Disease-relevant Pathways

Content Provider	Scilit
Author	Jung, Sung Yun Choi, Jong Min Rousseaux, Maxime W. C. Malovannaya, Anna Kim, Jean J. Kutzera, Joachim Wang, Yi Huang, Yin Zhu, Weimin Maity, Suman Zoghbi, Huda Yahya Qin, Jun
Copyright Year	2017
Description	Journal: Molecular & Cellular Proteomics Here, we present a mouse brain protein atlas that covers 17 surgically distinct neuroanatomical regions of the adult mouse brain, each less than 1 $mm^{3}$ in size. The protein expression levels are determined for 6,500 to 7,500 gene protein products from each region and over 12,000 gene protein products for the entire brain, documenting the physiological repertoire of mouse brain proteins in an anatomically resolved and comprehensive manner. We explored the utility of our spatially defined protein profiling methods in a mouse model of Parkinson's disease. We compared the proteome from a vulnerable region (substantia nigra pars compacta) of wild type and parkinsonian mice with that of an adjacent, less vulnerable, region (ventral tegmental area) and identified several proteins that exhibited both spatiotemporal- and genotype-restricted changes. We validated the most robustly altered proteins using an alternative profiling method and found that these modifications may highlight potential new pathways for future studies. This proteomic atlas is a valuable resource that offers a practical framework for investigating the molecular intricacies of normal brain function as well as regional vulnerability in neurological diseases. All of the mouse regional proteome profiling data are published on line at http://mbpa.bprc.ac.cn/.
Related Links	https://www.mcponline.org/content/mcprot/16/4/581.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383780/pdf http://www.mcponline.org/article/S1535947620324063/pdf
Ending Page	593
Page Count	13
Starting Page	581
ISSN	15359476
DOI	10.1074/mcp.m116.061440
Journal	Molecular & Cellular Proteomics
Issue Number	4
Volume Number	16
Language	English
Publisher	Elsevier BV
Publisher Date	2017-04-01
Access Restriction	Open
Subject Keyword	Journal: Molecular & Cellular Proteomics Biochemical Research
Content Type	Text
Resource Type	Article
Subject	Medicine Analytical Chemistry Molecular Biology Biochemistry

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