NDLI: Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions

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Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions

Content Provider	Scilit
Author	Hansen, Henning Gram Pristovšek, Nuša Kildegaard, Helene Faustrup Lee, Gyun Min
Copyright Year	2017
Description	Journal: Biotechnology Advances Chinese hamster ovary (CHO) cells are the preferred cell factory for the production of therapeutic glycoproteins. Although efforts primarily within bioprocess optimization have led to increased product titers of recombinant proteins (r-proteins) expressed in CHO cells, post-transcriptional bottlenecks in the biosynthetic pathway of r-proteins remain to be solved. To this end, the ectopic expression of transgenes (effector genes) offers great engineering potential. However, studies on effector genes have in some cases led to inconsistent results. Whereas this can in part be attributed to product specificity, other experimental and cellular factors are likely important contributors to these conflicting results. Here, these factors are reviewed and discussed with the objective of guiding future studies on effector genes.
Related Links	https://backend.orbit.dtu.dk/ws/files/130115282/Improving_the_secretory_capacity_222.pdf https://core.ac.uk/download/pdf/84002246.pdf
Ending Page	76
Page Count	13
Starting Page	64
ISSN	07349750
e-ISSN	18731899
DOI	10.1016/j.biotechadv.2016.11.008
Journal	Biotechnology Advances
Issue Number	1
Volume Number	35
Language	English
Publisher	Elsevier BV
Publisher Date	2017-01-01
Access Restriction	Open
Subject Keyword	Journal: Biotechnology Advances Biotechnology and Microbiology Cell Engineering Chinese Hamster Ovary (cho) Cells Er Stress Ectopic Expression Endoplasmic Reticulum Gene Dosage Product Quality Recombinant Protein Production Secretion Bottleneck Specific Productivity
Content Type	Text
Resource Type	Article
Subject	Bioengineering Applied Microbiology and Biotechnology Biotechnology

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Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions