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Compartmentation of Mitochondrial and Oxidative Metabolism in Growing Hair Follicles: A Ring of Fire
| Content Provider | Scilit |
|---|---|
| Author | Lemasters, John J. Ramshesh, Venkat K. Lovelace, Gregory L. Lim, John Wright, Graham D. Harland, Duane Dawson, Thomas L. |
| Copyright Year | 2017 |
| Description | Journal: Journal of Investigative Dermatology Little is known about the energetics of growing hair follicles, particularly in the mitochondrially abundant bulb. Here, mitochondrial and oxidative metabolism was visualized by multiphoton and light sheet microscopy in cultured bovine hair follicles and plucked human hairs. Mitochondrial membrane potential (ΔΨ), cell viability, reactive oxygen species (ROS), and secretory granules were assessed with parameter-indicating fluorophores. In growing follicles, lower bulb epithelial cells had high viability, and mitochondria were polarized. Most epithelially generated ROS co-localized with polarized mitochondria. As the imaging plane captured more central and distal cells, ΔΨ disappeared abruptly at a transition to a nonfluorescent core continuous with the hair shaft. Approaching the transition, ΔΨ and ROS increased, and secretory granules disappeared. ROS and ΔΨ were strongest in a circumferential paraxial ring at putative sites for formation of the outer cortex/cuticle of the hair shaft. By contrast, polarized mitochondria in dermal papillar fibroblasts produced minimal ROS. Plucked hairs showed a similar abrupt transition of degranulation/depolarization near sites of keratin deposition, as well as an ROS-generating paraxial ring of fire. Hair movement out of the follicle appeared to occur independently of follicular bulb bioenergetics by a tractor mechanism involving the inner and outer root sheaths. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5545130/pdf http://www.jidonline.org/article/S0022202X1731309X/pdf |
| Ending Page | 1444 |
| Page Count | 11 |
| Starting Page | 1434 |
| ISSN | 0022202X |
| e-ISSN | 15231747 |
| DOI | 10.1016/j.jid.2017.02.983 |
| Journal | Journal of Investigative Dermatology |
| Issue Number | 7 |
| Volume Number | 137 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2017-03-23 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Investigative Dermatology Mitochondrial Membrane Potential Chloromethyldihydrodichlorofluorescein Reactive Oxygen Species Tetramethylrhodamine Ethyl Ester Tetramethylrhodamine Methyl Ester |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Dermatology |
