NDLI: Aberrant determination of phenotypic markers in chronic lymphocytic leukemia (CLL) lymphocytes after cryopreservation

Please wait, while we are loading the content...

Aberrant determination of phenotypic markers in chronic lymphocytic leukemia (CLL) lymphocytes after cryopreservation

Content Provider	Scilit
Author	Thurgood, Lauren A. Lower, Karen M. Macardle, Cindy Kuss, Bryone J.
Copyright Year	2018
Description	Journal: Experimental hematology The cryopreservation of peripheral blood mononuclear cells (PBMCs) is a routine research laboratory process, enabling long-term storage of primary patient blood samples. Retrospective analysis of these samples has the potential to identify markers which may associate with prognosis and response to treatment. In order to draw valid biological conclusions from this type of analysis, it is essential to ensure that any observed changes are directly related to the pathology of the disease rather than the preservation process itself. Therefore, we have investigated 15 cell surface markers which are relevant to chronic lymphocytic leukemia (CLL) on matched fresh and thawed samples to determine the effect of cryopreservation on their detection. We found that the number of CLL cells positive for the markers CD22, CD40, CD49d, CD54, CD69 and CXCR3 was significantly decreased following cryopreservation. In addition, the MFI of 10 of the 15 markers significantly changed following cryopreservation. These findings demonstrate that care must be taken when interpreting this type of analysis on thawed samples.
Related Links	https://dspace.flinders.edu.au/xmlui/bitstream/2328/38169/1/Thurgood_Aberrant_AM2018.pdf
ISSN	0301472X
e-ISSN	18732399
DOI	10.1016/j.exphem.2018.04.003
Journal	Experimental hematology
Volume Number	63
Language	English
Publisher	Elsevier BV
Publisher Date	2018-07-01
Access Restriction	Open
Subject Keyword	Journal: Experimental hematology Chronic Lymphocytic Lymphocytic Leukemia
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Molecular Biology Hematology Cancer Research

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in