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SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area
| Content Provider | Scilit |
|---|---|
| Author | Randazzo, Walter Truchado, Pilar Cuevas-Ferrando, Enric Simón, Pedro Allende, Ana Sánchez, Gloria |
| Copyright Year | 2020 |
| Description | Journal: Water Research Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 200,000 reported COVID-19 cases in Spain resulting in more than 20,800 deaths as of April 21, 2020. Faecal shedding of SARS-CoV-2 RNA from COVID-19 patients has extensively been reported. Therefore, we investigated the occurrence of SARS-CoV-2 RNA in six wastewater treatments plants (WWTPs) serving the major municipalities within the Region of Murcia (Spain), the area with the lowest COVID-19 prevalence within Iberian Peninsula. Firstly, an aluminum hydroxide adsorption-precipitation concentration method was validated using a porcine coronavirus (Porcine Epidemic Diarrhea Virus, PEDV) and mengovirus (MgV). The procedure resulted in average recoveries of 10 ± 3.5% and 10 ± 2.1% in influent water (n = 2) and 3.3 ± 1.6% and 6.2 ± 1.0% in effluent water (n = 2) samples for PEDV and MgV, respectively. Then, the method was used to monitor the occurrence of SARS-CoV-2 from March 12 to April 14, 2020 in influent, secondary and tertiary effluent water samples. By using the real-time RT-PCR (RT-qPCR) Diagnostic Panel validated by US CDC that targets three regions of the virus nucleocapsid (N) gene, we estimated quantification of SARS-CoV-2 RNA titers in untreated wastewater samples of 5.4 ± 0.2 $log_{10}$ genomic copies/L on average. Two secondary water samples resulted positive (2 out of 18) and all tertiary water samples tested as negative (0 out 12). This environmental surveillance data were compared to declared COVID-19 cases at municipality level, revealing that members of the community were shedding SARS-CoV-2 RNA in their stool even before the first cases were reported by local or national authorities in many of the cities where wastewaters have been sampled. The detection of SARS-CoV-2 in wastewater in early stages of the spread of COVID-19 highlights the relevance of this strategy as an early indicator of the infection within a specific population. At this point, this environmental surveillance could be implemented by municipalities right away as a tool, designed to help authorities to coordinate the exit strategy to gradually lift its coronavirus lockdown. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229723/pdf |
| Ending Page | 115942 |
| Page Count | 1 |
| Starting Page | 115942 |
| ISSN | 00431354 |
| DOI | 10.1016/j.watres.2020.115942 |
| Journal | Water Research |
| Volume Number | 181 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2020-05-16 |
| Access Restriction | Open |
| Subject Keyword | Journal: Water Research Water Science and Technology Environmental Surveillance Influent Water Reclaimed Water Concentration Protocol Rna Virus |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ecological Modeling Waste Management and Disposal Pollution Environmental Engineering Water Science and Technology Civil and Structural Engineering |
