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Measurement of urinary Benzo[a]pyrene tetrols and their relationship to other polycyclic aromatic hydrocarbon metabolites and cotinine in humans
| Content Provider | Scilit |
|---|---|
| Author | Hilton, Donald C. Trinidad, Debra A. Hubbard, Kendra Li, Zheng Sjödin, Andreas |
| Copyright Year | 2017 |
| Description | Journal: Chemosphere Biomonitoring of exposure to polycyclic aromatic hydrocarbons (PAHs) typically uses measurement of metabolites of PAHs with four or less aromatic rings, such as 1-hydroxypyrene, even though interest may be in exposure to larger and carcinogenic PAHs, such as benzo[a]pyrene (B[a]P). An improved procedure for measuring two tetrol metabolites of B[a]P has been developed. Using 2 mL urine, the method includes enzymatic deconjugation of the tetrol conjugates, liquid-liquid extraction, activated carbon solid phase extraction (SPE) and Strata-X SPE, and gas chromatography–electron capture negative ionization–tandem mass spectrometric determination. Limits of detection were 0.026 pg/mL (benzo[a]pyrene-r-7,t-8,t-9,c-10-tetrahydrotetrol, BPT I-1) and 0.090 pg/mL (benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol, BPT II-1). We quantified BPT I-1 and BPT II-1 in urine from a volunteer who consumed one meal containing high levels of PAHs (barbequed chicken). We also measured urinary concentrations of BPT I-1 and BPT II-1 in smokers and nonsmokers, and compared these concentrations with those of monohydroxy PAHs (OH-PAHs) and cotinine. Urinary elimination of BPT I-1 and BPT II-1 as a function of time after dietary exposure was similar to that observed previously for OH-PAHs. While the median BPT I-1 concentration in smokers’ urine (0.069 pg/mL) significantly differs from nonsmokers (0.043 pg/mL), BPT I-1 is only weakly correlated with cotinine. The urinary concentration of BPT I-1 shows a weaker relationship to tobacco smoke than metabolites of smaller PAHs, suggesting that other routes of exposure such as for example dietary routes may be of larger quantitative importance. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647254/pdf |
| Ending Page | 372 |
| Page Count | 8 |
| Starting Page | 365 |
| ISSN | 00456535 |
| e-ISSN | 18791298 |
| DOI | 10.1016/j.chemosphere.2017.09.077 |
| Journal | Chemosphere |
| Volume Number | 189 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2017-09-18 |
| Access Restriction | Open |
| Subject Keyword | Journal: Chemosphere Analytical Chemistry Methylnaphthalene Polycyclic Aromatic Hydrocarbons |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Environmental Chemistry Health, Toxicology and Mutagenesis Pollution Public Health, Environmental and Occupational Health Environmental Engineering |
