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Biosensors for plant pathogen detection
| Content Provider | Scilit |
|---|---|
| Author | Khater, Mohga de la Escosura-Muñiz, Alfredo Merkoçi, Arben |
| Copyright Year | 2017 |
| Description | Journal: Biosensors and Bioelectronics Infectious plant diseases are caused by pathogenic microorganisms such as fungi, bacteria, viruses, viroids, phytoplasma and nematodes. Worldwide, plant pathogen infections are among main factors limiting crop productivity and increasing economic losses. Plant pathogen detection is important as first step to manage a plant disease in greenhouses, field conditions and at the country boarders. Current immunological techniques used to detect pathogens in plant include enzyme-linked immunosorbent assays (ELISA) and direct tissue blot immunoassays (DTBIA). DNA-based techniques such as polymerase chain reaction (PCR), real time PCR (RT-PCR) and dot blot hybridization have also been proposed for pathogen identification and detection. However these methodologies are time-consuming and require complex instruments, being not suitable for in-situ analysis. Consequently, there is strong interest for developing new biosensing systems for early detection of plant diseases with high sensitivity and specificity at the point-of-care. In this context, we revise here the recent advancement in the development of advantageous biosensing systems for plant pathogen detection based on both antibody and DNA receptors. The use of different nanomaterials such as nanochannels and metallic nanoparticles for the development of innovative and sensitive biosensing systems for the detection of pathogens (i.e. bacteria and viruses) at the point-of-care is also shown. Plastic and paper-based platforms have been used for this purpose, offering cheap and easy-to-use really integrated sensing systems for rapid on-site detection. Beside devices developed at research and development level a brief revision of commercially available kits is also included in this review.We acknowledge support from the Spanish MINECO under Project MAT2014-52485-P and through the Severo Ochoa Centers of Excellence Program under Grant SEV-2013-0295. The support from Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya (Grant 2014 SGR 260) is also acknowledged.Peer Reviewe |
| Related Links | https://ddd.uab.cat/pub/artpub/2017/192844/biobio_a2017v93p71.pdf |
| Ending Page | 86 |
| Page Count | 15 |
| Starting Page | 72 |
| ISSN | 09565663 |
| e-ISSN | 18734235 |
| DOI | 10.1016/j.bios.2016.09.091 |
| Journal | Biosensors and Bioelectronics |
| Volume Number | 93 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2017-07-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Biosensors and Bioelectronics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
