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Morphologic and immunophenotypical features distinguishing Merkel cell polyomavirus-positive and negative Merkel cell carcinoma
| Content Provider | Scilit |
|---|---|
| Author | Kervarrec, Thibault Tallet, Anne Miquelestorena-Standley, Elodie Houben, Roland Schrama, David Gambichler, Thilo Berthon, Patricia Corre, Yannick Le Hainaut-Wierzbicka, Ewa Aubin, Francois Bens, Guido Tabareau-Delalande, Flore Beneton, Nathalie Fromont, Gaëlle Arbion, Flavie Leteurtre, Emmanuelle Herfs, Michael Touzé, Antoine Samimi, Mahtab Guyétant, Serge |
| Copyright Year | 2019 |
| Description | Journal: Modern Pathology In 2008, Feng et al. identified Merkel cell polyomavirus integration as the primary oncogenic event in ~80% of Merkel cell carcinoma cases. The remaining virus-negative Merkel cell carcinoma cases associated with a high mutational load are most likely caused by UV radiation. The current study aimed to compare the morphological and immunohistochemical features of 80 virus-positive and 21 virus-negative Merkel cell carcinoma cases. Microscopic evaluation revealed that elongated nuclei—similar to the spindle-shape variant of small cell lung cancer—were less frequent in Merkel cell polyomavirus-positive Merkel cell carcinoma compared to the virus-negative subset (p = 0.005). Moreover, virus-negative cases more frequently displayed a “large-cell neuroendocrine carcinoma” phenotype with larger cell size (p = 0.0026), abundant cytoplasm (p = $4×10^{−7}$) and prominent nucleoli (p = 0.002). Analysis of immunohistochemical data revealed frequent positivity for thyroid transcription factor 1 and cytokeratin 7, either absence or overexpression of p53, as well as frequent lack of neurofilament expression in virus-negative cases. By contrast, cytokeratin 8, 18 and 20 and a CD99 with a dot pattern as well as high EMA expression were identified as characteristic features of virus-positive Merkel cell carcinoma. In particular, the CD99 dot-like expression pattern was strongly associated with presence of the Merkel cell polyomavirus in Merkel cell carcinoma (sensitivity = 81%, specificity = 90%, positive likelihood ratio = 8.08). To conclude, virus-positive and -negative Merkel cell carcinoma are characterized by distinct morphological and immunohistochemical features, which implies a significant difference in tumor biology and behavior. Importantly, we identified the CD99 staining pattern as a marker indicating the virus status of this skin cancer. |
| Related Links | https://www.nature.com/articles/s41379-019-0288-7.pdf |
| Ending Page | 1616 |
| Page Count | 12 |
| Starting Page | 1605 |
| ISSN | 08933952 |
| e-ISSN | 15300285 |
| DOI | 10.1038/s41379-019-0288-7 |
| Journal | Modern Pathology |
| Issue Number | 11 |
| Volume Number | 32 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2019-06-14 |
| Access Restriction | Open |
| Subject Keyword | Journal: Modern Pathology Neuroendocrine Cancer Skin Cancer Medicine/public Health Laboratory Medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pathology and Forensic Medicine |
