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The effects of apolipoprotein B depletion on HDL subspecies composition and function
| Content Provider | Scilit |
|---|---|
| Author | Davidson, W. Sean Heink, Anna Sexmith, Hannah Melchior, John T. Gordon, Scott M. Kuklenyik, Zsuzsanna Woollett, Laura Barr, John R. Jones, Jeffrey I. Toth, Christopher A. Shah, Amy S. |
| Copyright Year | 2016 |
| Description | Journal: Journal of Lipid Research High density lipoprotein (HDL) cholesterol efflux function may be a more robust biomarker of coronary artery disease risk than HDL cholesterol (HDL-C). To study HDL function, apoB containing lipoproteins are precipitated from serum. Whether apoB precipitation affects HDL subspecies composition and function has not been thoroughly investigated. We studied the effects of four common apoB precipitation methods (polyethylene glycol (PEG), dextran sulfate/MgCl2, heparin sodium/MnCl2 and LipoSep immunoprecipitant (IP)) on HDL subspecies composition, apolipoproteins and function (cholesterol efflux and reduction of LDL oxidation). PEG dramatically shifted the size distribution of HDL and apolipoproteins (assessed by two independent methods), while leaving substantial amounts of reagent in the sample. PEG also changed the distribution of cholesterol efflux and LDL oxidation across size fractions, but not overall efflux across the HDL range. Dextran sulfate/MgCl2 and heparin sodium/MnCl2 did not change the size distribution of HDL subspecies but altered the quantity of a subset of apolipoproteins. LipoSep IP resulted in a shift in the HDL size distribution, but less so than PEG. Thus, each of the apoB precipitation methods affected HDL composition and/or size distribution. We conclude that careful evaluation is needed when selecting apoB depletion methods for existing and future bioassays of HDL function. |
| Related Links | http://www.jlr.org/content/57/4/674.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808776/pdf http://www.jlr.org/content/early/2016/02/23/jlr.M066613.full.pdf |
| Ending Page | 686 |
| Page Count | 13 |
| Starting Page | 674 |
| ISSN | 00222275 |
| e-ISSN | 15397262 |
| DOI | 10.1194/jlr.m066613 |
| Journal | Journal of Lipid Research |
| Issue Number | 4 |
| Volume Number | 57 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2016-04-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Lipid Research Medical Laboratory Technology Cholesterol Efflux High Density Lipoprotein Low Density Lipoprotein Polyethylene Glycol |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Endocrinology |
