Loading...
Please wait, while we are loading the content...
Similar Documents
Promyelocytic Leukemia Protein (PML) Requirement for Interferon-induced Global Cellular SUMOylation
| Content Provider | Scilit |
|---|---|
| Author | Maroui, Mohamed Ali Maarifi, Ghizlane McManus, Francis P. Lamoliatte, Frédéric Thibault, Pierre Chelbi-Alix, Mounira K. |
| Copyright Year | 2018 |
| Description | Journal: Molecular & Cellular Proteomics We report that interferon (IFN) a treatment at short and long periods increases the global cellular SUMOylation and requires the presence of the SUMO E3 ligase promyelocytic leukemia protein (PML), the organizer of PML nuclear bodies (NBs). Several PML isoforms (PMLI-PMLVII) derived from a single PML gene by alternative splicing, share the same N-terminal region but differ in their C-terminal sequences. Introducing each of the human PML isoform in PML-negative cells revealed that enhanced SUMOylation in response to IFN is orchestrated by PMLIII and PMLIV. Large-scale proteomics experiments enabled the identification of 558 SUMO sites on 389 proteins, of which 172 sites showed differential regulation upon IFNa stimulation, including K49 from UBC9, the sole SUMO E2 protein. Furthermore, IFNa induces PML-dependent UBC9 transfer to the nuclear matrix where it colocalizes with PML within the NBs and enhances cellular SUMOylation levels. Our results demonstrate that SUMOylated UBC9 and PML are key players for IFN-increased cellular SUMOylation. |
| Related Links | https://www.mcponline.org/content/mcprot/17/6/1196.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986244/pdf http://www.mcponline.org/content/early/2018/03/13/mcp.RA117.000447.full.pdf |
| Ending Page | 1208 |
| Page Count | 13 |
| Starting Page | 1196 |
| ISSN | 15359476 |
| DOI | 10.1074/mcp.ra117.000447 |
| Journal | Molecular & Cellular Proteomics |
| Issue Number | 6 |
| Volume Number | 17 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2018-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Molecular & Cellular Proteomics Biochemical Research Mass Spectrometry Post-translational Modifications* Tandem Mass Spectrometry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Analytical Chemistry Molecular Biology Biochemistry |
