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A biomimetic synthetic feeder layer supports the proliferation and self-renewal of mouse embryonic stem cells
| Content Provider | Scilit |
|---|---|
| Author | López-Fagundo, Cristina Livi, Liane L. Ramchal, Talisha Darling, Eric M. Hoffman-Kim, Diane |
| Copyright Year | 2016 |
| Description | Journal: Acta biomaterialia Successful realization of the enormous potential of pluripotent stem cells in regenerative medicine demands the development of well-defined culture conditions. Maintenance of embryonic stem cells (ESCs) typically requires co-culture with feeder layer cells, generally mouse embryonic fibroblasts (MEFs). Concerns about xenogeneic pathogen contamination and immune reaction to feeder cells underlie the need for ensuring the safety and efficacy of future stem cell-based products through the development of a controlled culture environment. To gain insight into the effectiveness of MEF layers, here we have developed a biomimetic synthetic feeder layer (BSFL) that is acellular and replicates the stiffness and topography of MEFs. The mechanical properties of MEFs were measured using atomic force microscopy. The average Young’s modulus of the MEF monolayers was replicated using tunable polyacrylamide (PA) gels. BSFLs replicated topographical features of the MEFs, including cellular, subcellular, and cytoskeletal features. On BSFLs, mouse ESCs adhered and formed compact round colonies; similar to on MEF controls but not on Flat PA. ESCs on BSFLs maintained their pluripotency and self-renewal across passages, formed embryoid bodies and differentiated into progenitors of the three germ layers. This acellular biomimetic synthetic feeder layer supports stem cell culture without requiring co-culture of live xenogeneic feeder cells, and provides a versatile, tailorable platform for investigating stem cell growth. |
| Related Links | http://europepmc.org/articles/pmc4905775?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905775/pdf |
| Ending Page | 64 |
| Page Count | 10 |
| Starting Page | 55 |
| ISSN | 17427061 |
| e-ISSN | 18787568 |
| DOI | 10.1016/j.actbio.2016.04.047 |
| Journal | Acta biomaterialia |
| Volume Number | 39 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2016-04-30 |
| Access Restriction | Open |
| Subject Keyword | Journal: Acta biomaterialia Embryonic Stem Cells Mouse Embryonic Fibroblast |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biochemistry Molecular Biology Biomaterials Biomedical Engineering Biotechnology |
