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Effect of storage temperature on the stability of spray dried bacteriophage powders
| Content Provider | Scilit |
|---|---|
| Author | Leung, Sharon S. Y. Parumasivam, Thaigarajan Nguyen, An Gengenbach, Thomas Carter, Elizabeth A. Carrigy, Nicholas B. Wang, Hui Vehring, Reinhard Finlay, Warren H. Morales, Sandra Britton, Warwick J. Kutter, Elizabeth Chan, Hak-Kim |
| Copyright Year | 2018 |
| Description | Journal: European Journal of Pharmaceutics and Biopharmaceutics This study aimed to assess the robustness of using a spray drying approach and formulation design in producing inhalable phage powders. Two types of Pseudomonas phages, PEV2 (Podovirus) and PEV40 (Myovirus) in two formulations containing different amounts of trehalose (70% and 60%) and leucine (30% and 40%) were studied. Most of the surface of the produced powders was found to be covered in crystalline leucine. The powders were stored at 4 °C and 20 °C under vacuum. The phage stability and in vitro aerosol performance of the phage powders were examined on the day of production and after 1, 3 and 12 months of storage. A minor titer loss during production was observed for both phages (0.2–0.8 $log_{10}$ pfu/ml). The storage stability of the produced phage powders was found to be phage and formulation dependent. No further reduction in titer occurred for PEV2 powders stored at 4 °C across the study. The formulation containing 30% leucine maintained the viability of PEV2 at 20 °C, while the formulation containing 40% leucine gradually lost titer over time with a storage reduction of ∼0.9 $log_{10}$ pfu/ml measured after 12 months. In comparison, the PEV40 phage powders generally had a ∼ 0.5 $log_{10}$ pfu/ml loss upon storage regardless of temperature. When aerosolized, the total in vitro lung doses of PEV2 were of the order of $10^{7}$ pfu, except the formulation containing 40% leucine stored at 20 °C which had a lower lung dose. The PEV40 powders also had lung doses of $10^{6}–10^{7}$ pfu. The results demonstrate that spray dried Myoviridae and Podoviridae phage in a simple formulation of leucine and trehalose can be successfully stored for one year at 4 °C and 20 °C with vacuum packaging. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948144/pdf |
| Ending Page | 222 |
| Page Count | 10 |
| Starting Page | 213 |
| ISSN | 09396411 |
| DOI | 10.1016/j.ejpb.2018.02.033 |
| Journal | European Journal of Pharmaceutics and Biopharmaceutics |
| Volume Number | 127 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2018-02-24 |
| Access Restriction | Open |
| Subject Keyword | Journal: European Journal of Pharmaceutics and Biopharmaceutics Pharmacology and Pharmacy Pulmonary Infections Phage Dry Powder Antibiotic Resistance |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biotechnology Pharmaceutical Science |
