Loading...
Please wait, while we are loading the content...
Similar Documents
Pore size directs bone marrow stromal cell fate and tissue regeneration in nanofibrous macroporous scaffolds by mediating vascularization
| Content Provider | Scilit |
|---|---|
| Author | Gupte, Melanie J. Swanson, W. Benton Hu, Jiang Jin, Xiaobing Ma, Haiyun Zhang, Zhanpeng Liu, Zhongning Feng, Kai Feng, Ganjun Xiao, Guiyong Hatch, Nan Mishina, Yuji Ma, Peter X. |
| Copyright Year | 2018 |
| Description | Journal: Acta biomaterialia In the U.S., 30% of adults suffer joint pain, most commonly in the knee, which severely limits mobility and is often attributed to injury of cartilage and underlying bone in the joint. Current treatment methods such as microfracture result in less resilient fibrocartilage with eventual failure; autografting can cause donor site morbidity and poor integration. To overcome drawbacks in treatment, tissue engineers can design cell-instructive biomimetic scaffolds using biocompatible materials as alternate therapies for osteochondral defects. Nanofibrous poly (l-lactic acid) (PLLA) scaffolds of uniform, spherical, interconnected and well-defined pore sizes that are fabricated using a thermally-induced phase separation and sugar porogen template method create an extracellular matrix-like environment which facilitates cell adhesion and proliferation. Herein we report that chondrogenesis and endochondral ossification of rabbit and human bone marrow stromal cells (BMSCs) can be controlled by scaffold pore architecture, particularly pore size. Small-pore scaffolds support enhanced chondrogenic differentiation in vitro and cartilage formation in vivo compared to large-pore scaffolds. Endochondral ossification is prevented in scaffolds with very small pore sizes; pore interconnectivity is critical to promote capillary ingrowth for mature bone formation. These results provide a novel strategy to control tissue regenerative processes by tunable architecture of macroporous nanofibrous scaffolds. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258662/pdf |
| ISSN | 17427061 |
| e-ISSN | 18787568 |
| DOI | 10.1016/j.actbio.2018.10.016 |
| Journal | Acta biomaterialia |
| Volume Number | 82 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2018-10-13 |
| Access Restriction | Open |
| Subject Keyword | Journal: Acta biomaterialia Bone Marrow Stromal Cells Bone Tissue Engineering Cartilage Tissue Engineering |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biochemistry Molecular Biology Biomaterials Biomedical Engineering Biotechnology |
