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Proteome Dynamics: Tissue Variation in the Kinetics of Proteostasis in Intact Animals
| Content Provider | Scilit |
|---|---|
| Author | Hammond, Dean E. Claydon, Amy J. Simpson, Deborah M. Edward, Dominic Stockley, Paula Hurst, Jane L. Beynon, Robert J. |
| Copyright Year | 2016 |
| Description | Journal: Molecular & Cellular Proteomics Understanding the role of protein turnover in the maintenance of proteostasis requires accurate measurements of the rates of replacement of proteins in complex systems, such as intact animals. Moreover, any investigation of allometric scaling of protein turnover is likely to include species for which fully annotated proteomes are not available. We have used dietary administration of stable isotope labeled lysine to assess protein turnover rates for proteins from four tissues in the bank vole, Myodes glareolus. The annotated genome for this species is not available, so protein identification was attained through cross-species matching to the mouse. For proteins for which confident identifications were derived, the pattern of lysine incorporation over 40 days was used to define the rate of synthesis of individual proteins in the four tissues. The data were heavily filtered to retain a very high quality dataset of turnover rates for 1088 proteins. Comparative analysis of the four tissues revealed different median rates of degradation (kidney: 0.099 $days^{−1}$; liver 0.136 $days^{−1}$; heart, 0.054 $days^{−1}$, and skeletal muscle, 0.035 $days^{−1}$). These data were compared with protein degradation rates from other studies on intact animals or from cells in culture and indicate that both cell type and analytical methodology may contribute to variance in turnover data between different studies. These differences were not only due to tissue-specific proteins but were reflected in gene products common to all tissues. All data are available via ProteomeXchange with identifier PXD002054. |
| Related Links | https://www.mcponline.org/content/mcprot/15/4/1204.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824850/pdf http://www.mcponline.org/article/S1535947620336161/pdf |
| Ending Page | 1219 |
| Page Count | 16 |
| Starting Page | 1204 |
| ISSN | 15359476 |
| DOI | 10.1074/mcp.m115.053488 |
| Journal | Molecular & Cellular Proteomics |
| Issue Number | 4 |
| Volume Number | 15 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2016-04-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Molecular & Cellular Proteomics Biochemical Research Cells in Culture Intact Animals Protein Turnover |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Analytical Chemistry Molecular Biology Biochemistry |
