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MS-READ: Quantitative measurement of amino acid incorporation
| Content Provider | Scilit |
|---|---|
| Author | Mohler, Kyle Aerni, Hans-Rudolf Gassaway, Brandon Ling, Jiqiang Ibba, Michael Rinehart, Jesse |
| Copyright Year | 2017 |
| Description | Journal: Biochimica et Biophysica Acta (BBA) - General Subjects Ribosomal protein synthesis results in the genetically programmed incorporation of amino acids into a growing polypeptide chain. Faithful amino acid incorporation that accurately reflects the genetic code is critical to the structure and function of proteins as well as overall proteome integrity. Errors in protein synthesis are generally detrimental to cellular processes yet emerging evidence suggest that proteome diversity generated through mistranslation may be beneficial under certain conditions. Cumulative translational error rates have been determined at the organismal level, however codon specific error rates and the spectrum of misincorporation errors from system to system remain largely unexplored. In particular, until recently technical challenges have limited the ability to detect and quantify comparatively rare amino acid misincorporation events, which occur orders of magnitude less frequently than canonical amino acid incorporation events. We now describe a technique for the quantitative analysis of amino acid incorporation that provides the sensitivity necessary to detect mistranslation events during translation of a single codon at frequencies as low as 1 in 10,000 for all 20 proteinogenic amino acids, as well as non-proteinogenic and modified amino acids. This article is part of a Special Issue entitled "Biochemistry of Synthetic Biology - Recent Developments" Guest Editor: Dr. Ilka Heinemann and Dr. Patrick O'Donoghue. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524608/pdf |
| Ending Page | 3088 |
| Page Count | 8 |
| Starting Page | 3081 |
| ISSN | 03044165 |
| DOI | 10.1016/j.bbagen.2017.01.025 |
| Journal | Biochimica et Biophysica Acta (BBA) - General Subjects |
| Issue Number | 11 |
| Volume Number | 1861 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2017-11-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Biochimica et Biophysica Acta (BBA) - General Subjects Biochemical Research Mass Spectrometry Reporter |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Biochemistry Biophysics |
