NDLI: Role of phospholipase D in the cAMP signal transduction pathway activated during fibroblast contraction of collagen matrices.

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Role of phospholipase D in the cAMP signal transduction pathway activated during fibroblast contraction of collagen matrices.

Content Provider	Scilit
Author	He, Y. Grinnell, F.
Copyright Year	1995
Description	Journal: Journal of Cell Biology Fibroblast contraction of stressed collagen matrices results in activation of a cAMP signal transduction pathway. This pathway involves influx of extracellular Ca2+ ions and increased production of arachidonic acid. We report that within 5 min after initiating contraction, a burst of phosphatidic acid release was detected. Phospholipase D was implicated in production of phosphatidic acid based on observation of a transphosphatidylation reaction in the presence of ethanol that resulted in formation of phosphatidylethanol at the expense of phosphatidic acid. Activation of phospholipase D required extracellular Ca2+ ions and was regulated by protein kinase C. Ethanol treatment of cells also inhibited by 60-70% contraction-dependent release of arachidonic acid and cAMP but had no effect on increased cAMP synthesis after addition of exogenous arachidonic acid or on phospholipase A2 activity measured in cell extracts. Moreover, other treatments that inhibited the burst of phosphatidic acid release after contraction--chelating extracellular Ca2+ or down-regulating protein kinase C--also blocked contraction activated cyclic AMP signaling. These results were consistent with the idea that phosphatidic acid production occurred upstream of arachidonic acid in the contraction-activated cAMP signaling pathway.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120549/pdf http://jcb.rupress.org/content/130/5/1197.full.pdf
ISSN	00221295
DOI	10.1083/jcb.130.5.1197
Journal	Journal of Cell Biology
Issue Number	5
Volume Number	130
Language	English
Publisher	Rockefeller University Press
Publisher Date	1995-09-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Cell Biology Biochemistry and Molecular Biology Phospholipase Phosphatidic Acid Arachidonic Acid
Content Type	Text
Resource Type	Article
Subject	Physiology

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