NDLI: THE NUCLEIC ACIDS OF BASAL BODIES ISOLATED FROM TETRAHYMENA PYRIFORMIS

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THE NUCLEIC ACIDS OF BASAL BODIES ISOLATED FROM TETRAHYMENA PYRIFORMIS

Content Provider	Scilit
Author	Hoffman, Eugene J.
Copyright Year	1965
Description	Pellicular fragments were isolated from ethanol-fixed cells of the holotrichous ciliate Tetrahymena pyriformis by the action of digitonin. The isolated pellicles were further fragmented and the basal bodies of the cilia isolated from them by three methods. The preparations, examined in the electron microscope as embedded sections or negatively stained samples, consisted mainly of somewhat deformed pellicular material, the bulk of which was basal body. DNA was determined by the diphenylamine method and by reaction with DNase, and RNA, by the orcinol method. Nucleic acids were isolated by phenol extraction and analyzed spectrophotometrically and by reaction with RNase. The assays indicated 1.2 to 2.6 per cent RNA, similar to previously published work, but only 0.0 to 1.0 per cent DNA, near enough the sensitivity limits to render the presence of DNA in the preparations uncertain. Although the isolation procedure removed nuclear contents and ribosomes, the nucleic acids could still be a residual contaminant bound to the pellicle during the isolation. Hypotheses of basal body self-duplication, moreover, can be constructed both with and without nucleic acids.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106658/pdf http://jcb.rupress.org/content/25/2/217.full.pdf
Ending Page	228
Page Count	12
Starting Page	217
DOI	10.1083/jcb.25.2.217
Journal	Journal of Cell Biology
Issue Number	2
Volume Number	25
Language	English
Publisher	Rockefeller University Press
Publisher Date	1965-05-01
Access Restriction	Open
Subject Keyword	Anesthesiology Cilia Cytology Deoxyribonuclease Digitalis Glycosides Dna Experimental Lab Study Microscopy, Electron Ribonuclease Ribosomes Rna Spectrophotometry Tetrahymena Journal: Journal of Cell Biology (Vol- 25, Issue- 2)
Content Type	Text

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