NDLI: A novel role of farnesylation in targeting a mitotic checkpoint protein, human Spindly, to kinetochores

Please wait, while we are loading the content...

A novel role of farnesylation in targeting a mitotic checkpoint protein, human Spindly, to kinetochores

Content Provider	Scilit
Author	Moudgil, Devinderjit K. Westcott, Nathan Famulski, Jakub K. Patel, Kinjal Macdonald, Dawn Hang, Howard Chan, Gordon K. T.
Copyright Year	2015
Description	Kinetochore (KT) localization of mitotic checkpoint proteins is essential for their function during mitosis. hSpindly KT localization is dependent on the RZZ complex and hSpindly recruits the dynein–dynactin complex to KTs during mitosis, but the mechanism of hSpindly KT recruitment is unknown. Through domain-mapping studies we characterized the KT localization domain of hSpindly and discovered it undergoes farnesylation at the C-terminal cysteine residue. The N-terminal 293 residues of hSpindly are dispensable for its KT localization. Inhibition of farnesylation using a farnesyl transferase inhibitor (FTI) abrogated hSpindly KT localization without affecting RZZ complex, CENP-E, and CENP-F KT localization. We showed that hSpindly is farnesylated in vivo and farnesylation is essential for its interaction with the RZZ complex and hence KT localization. FTI treatment and hSpindly knockdown displayed the same mitotic phenotypes, indicating that hSpindly is a key FTI target in mitosis. Our data show a novel role of lipidation in targeting a checkpoint protein to KTs through protein–protein interaction.
Related Links	http://jcb.rupress.org/content/jcb/208/7/881.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384735/pdf http://jcb.rupress.org/content/208/7/881.full.pdf
Ending Page	896
Page Count	16
Starting Page	881
DOI	10.1083/jcb.201412085
Journal	Journal of Cell Biology
Issue Number	7
Volume Number	208
Language	English
Publisher	Rockefeller University Press
Publisher Date	2015-03-30
Access Restriction	Open
Subject Keyword	Cell Biology Farnesylation Mitosis Localization Hspindly Kt Rzz Complex Checkpoint Protein Terminal Journal: Journal of Cell Biology (Vol- 208, Issue- 7)
Content Type	Text

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in