NDLI: Distribution of acetylated alpha-tubulin in Physarum polycephalum.

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Distribution of acetylated alpha-tubulin in Physarum polycephalum.

Content Provider	Scilit
Author	Diggins, M. A. Dove, W. F.
Copyright Year	1987
Description	Journal: Journal of Cell Biology The expression and cytological distribution of acetylated alpha-tubulin was investigated in Physarum polycephalum. A monoclonal antibody specific for acetylated alpha-tubulin, 6-11B-1 (Piperno, G., and M. T. Fuller, 1985, J. Cell Biol., 101:2085-2094), was used to screen for this protein during three different stages of the Physarum life cycle--the amoeba, the flagellate, and the plasmodium. Western blots of two-dimensional gels of amoebal and flagellate proteins reveal that this antibody recognizes the alpha 3 tubulin isotype, which was previously shown to be formed by posttranslational modification (Green, L. L., and W. F. Dove, 1984, Mol. Cell. Biol., 4:1706-1711). Double-label immunofluorescence demonstrates that, in the flagellate, acetylated alpha-tubulin is localized in the flagella and flagellar cone. Similar experiments with amoebae interestingly reveal that only within the microtubule organizing center (MTOC) are there detectable amounts of acetylated alpha-tubulin. In contrast, the plasmodial stage gives no evidence for acetylated alpha-tubulin by Western blotting or by immunofluorescence.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114406/pdf http://jcb.rupress.org/content/104/2/303.full.pdf
ISSN	00221295
DOI	10.1083/jcb.104.2.303
Journal	Journal of Cell Biology
Issue Number	2
Volume Number	104
Language	English
Publisher	Rockefeller University Press
Publisher Date	1987-02-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Cell Biology Parasitology Polycephalum Acetylated Alpha Tubulin
Content Type	Text
Resource Type	Article
Subject	Physiology

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