NDLI: Phosphorylation of nuclear and flagellar basal apparatus proteins during flagellar regeneration in Chlamydomonas reinhardtii

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Phosphorylation of nuclear and flagellar basal apparatus proteins during flagellar regeneration in Chlamydomonas reinhardtii

Content Provider	Scilit
Author	Harper, J. D. Sanders, M. A. Salisbury, Jeffrey
Copyright Year	1993
Description	The antiphosphoprotein monoclonal antibody MPM-2 was used to investigate protein phosphorylation during flagellar regeneration in Chlamydomonas reinhardtii. MPM-2 recognizes a phosphorylated epitope and detects several Chlamydomonas proteins by Western immunoblot analysis. Two MPM-2 reactive proteins (34 and 90 kD) increase in Western immunoblot intensity after flagellar excision and decrease in intensity during flagellar regeneration. Immunofluorescence and immunogold labeling revealed MPM-2 staining within the nucleus, especially towards the nuclear periphery, the flagellar basal apparatus, and the nucleus-basal body connector after flagellar excision. Comparison of MPM-2 reactivity in wild-type cells and in the mutant bald-2, which lacks functional basal bodies, demonstrates that the 34-kD protein is localized in the nucleus and the 90-kD protein is localized in the flagellar basal region. MPM-2 reactivity is observed in cells competent for flagellar regeneration. However, when cells were treated with the kinase inhibitor, staurosporine, MPM-2 reactivity did not increase after flagellar excision and flagellar regeneration was impaired. These observations suggest that phosphorylation of the 34- and 90-kD proteins may be important for flagellar regrowth. Possible roles for phosphorylation in flagellar regeneration include transcriptional activation and transport of flagellar precursors to the base of the growing flagella.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119581/pdf http://jcb.rupress.org/content/122/4/877.full.pdf
Ending Page	886
Page Count	10
Starting Page	877
DOI	10.1083/jcb.122.4.877
Journal	Journal of Cell Biology
Issue Number	4
Volume Number	122
Language	English
Publisher	Rockefeller University Press
Publisher Date	1993-08-15
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Antibody Phosphorylation Proteins Immunoblot Mpm Western Basal Flagellar Regeneration Journal: Journal of Cell Biology (Vol- 122, Issue- 4)
Content Type	Text

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