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Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy.
| Content Provider | Scilit |
|---|---|
| Author | Heggeness, Michael H. Ash, J. F. |
| Copyright Year | 1977 |
| Description | A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM, which binds to actin, or the biotinyl antibody, which binds to myosin. In a second step the cell are treated with a fluorescent derivative of avidin (Fl-avidin) which binds to the biotinyl proteins and thus indirectly reveals the location of the cellular action or myosin. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111432/pdf http://jcb.rupress.org/content/73/3/783.full.pdf |
| Ending Page | 788 |
| Page Count | 6 |
| Starting Page | 783 |
| DOI | 10.1083/jcb.73.3.783 |
| Journal | Journal of Cell Biology |
| Issue Number | 3 |
| Volume Number | 73 |
| Language | English |
| Publisher | Rockefeller University Press |
| Publisher Date | 1977-06-01 |
| Access Restriction | Open |
| Subject Keyword | Analytical Chemistry Fluorescence Microscopy Journal: Journal of Cell Biology (Vol- 73, Issue- 3) |
| Content Type | Text |