NDLI: ARHGEF17 is an essential spindle assembly checkpoint factor that targets Mps1 to kinetochores

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ARHGEF17 is an essential spindle assembly checkpoint factor that targets Mps1 to kinetochores

Content Provider	Scilit
Author	Isokane, Mayumi Walter, Thomas Mahen, Robert Nijmeijer, Bianca Hériché, Jean-Karim Miura, Kota Maffini, Stefano Ivanov, Miroslav Penchev Kitajima, Tomoya S. Peters, Jan-Michael Ellenberg, Jan
Copyright Year	2016
Description	To prevent genome instability, mitotic exit is delayed until all chromosomes are properly attached to the mitotic spindle by the spindle assembly checkpoint (SAC). In this study, we characterized the function of ARHGEF17, identified in a genome-wide RNA interference screen for human mitosis genes. Through a series of quantitative imaging, biochemical, and biophysical experiments, we showed that ARHGEF17 is essential for SAC activity, because it is the major targeting factor that controls localization of the checkpoint kinase Mps1 to the kinetochore. This mitotic function is mediated by direct interaction of the central domain of ARHGEF17 with Mps1, which is autoregulated by the activity of Mps1 kinase, for which ARHGEF17 is a substrate. This mitosis-specific role is independent of ARHGEF17’s RhoGEF activity in interphase. Our study thus assigns a new mitotic function to ARHGEF17 and reveals the molecular mechanism for a key step in SAC establishment.
Related Links	https://rupress.org/jcb/article-pdf/212/6/647/955249/jcb_201408089.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792069/pdf http://jcb.rupress.org/content/212/6/647.full.pdf
Ending Page	659
Page Count	13
Starting Page	647
DOI	10.1083/jcb.201408089
Journal	Journal of Cell Biology
Issue Number	6
Volume Number	212
Language	English
Publisher	Rockefeller University Press
Publisher Date	2016-03-07
Access Restriction	Open
Subject Keyword	Cell Biology Mps1 Spindle Mitotic Checkpoint Function of Arhgef17 Kinase Assembly Sac Journal: Journal of Cell Biology (Vol- 212, Issue- 6)
Content Type	Text

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