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Radical cure of experimental babesiosis in immunodeficient mice using a combination of an endochin-like quinolone and atovaquone
| Content Provider | Scilit |
|---|---|
| Author | Lawres, Lauren A. Garg, Aprajita Kumar, Vidya Bruzual, Igor Forquer, Isaac P. Renard, Isaline Virji, Azan Z. Boulard, Pierre Rodriguez, Eduardo X. Allen, Alexander J. Pou, Sovitj Wegmann, Keith W. Winter, Rolf W. Nilsen, Aaron Mao, Jialing Preston, Douglas A. Belperron, Alexia A. Bockenstedt, Linda K. Hinrichs, David J. Riscoe, Michael K. Doggett, J. Stone Mamoun, Choukri Ben |
| Copyright Year | 2016 |
| Description | Human babesiosis is a tick-borne multisystem disease caused by Babesia species of the apicomplexan phylum. Most clinical cases and fatalities of babesiosis are caused by Babesia microti. Current treatment for human babesiosis consists of two drug combinations, atovaquone + azithromycin or quinine + clindamycin. These treatments are associated with adverse side effects and a significant rate of drug failure. Here, we provide evidence for radical cure of experimental babesiosis in immunodeficient mice using a combination of an endochin-like quinolone (ELQ) prodrug and atovaquone. In vivo efficacy studies in mice using ELQ-271, ELQ-316, and the ELQ-316 prodrug, ELQ-334, demonstrated excellent growth inhibitory activity against the parasite, with potency equal to that of orally administered atovaquone at 10 mg/kg. Analysis of recrudescent parasites after ELQ or atovaquone monotherapy identified genetic substitutions in the Qi or Qo sites, respectively, of the cytochrome bc1 complex. Impressively, a combination of ELQ-334 and atovaquone, at doses as low as 5.0 mg/kg each, resulted in complete clearance of the parasite with no recrudescence up to 122 d after discontinuation of therapy. These results will set the stage for future clinical evaluation of ELQ and atovaquone combination therapy for treatment of human babesiosis. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925016/pdf http://jem.rupress.org/content/early/2016/06/06/jem.20151519.full.pdf |
| Ending Page | 1318 |
| Page Count | 12 |
| Starting Page | 1307 |
| DOI | 10.1084/jem.20151519 |
| Journal | The Journal of experimental medicine |
| Issue Number | 7 |
| Volume Number | 213 |
| Language | English |
| Publisher | Rockefeller University Press |
| Publisher Date | 2016-06-06 |
| Access Restriction | Open |
| Subject Keyword | Parasitology Treatment Efficacy Mice Babesiosis Parasite Prodrug Atovaquone Babesia Mg/kg Elq Journal: The Journal of experimental medicine (Vol- 213, Issue- 7) |
| Content Type | Text |
