NDLI: Human platelet activation by C3a and C3a des-arg.

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Human platelet activation by C3a and C3a des-arg.

Content Provider	Scilit
Author	Polley, M. J. Nachman, R. L.
Copyright Year	1983
Description	Journal: The Journal of experimental medicine C3a liberated from C3 by treatment with C3 convertase (or by trypsin) induced aggregation of gel-filtered human platelets and stimulated serotonin release. At concentrations of 10(-10) M to 8 X 10(-12) M, C3a induced aggregation when added alone to platelets. However, at lower concentrations (2 X 10(-12) M) C3a did not aggregate platelets directly but exhibited highly significant synergism (two-way analysis of variance P less than 0.0001) with ADP in mediating platelet aggregation and release of serotonin. Removal of the C-terminus arginine from C3a abolished anaphylotoxin activity but did not affect the platelet-stimulating activity of the peptide. C3a and C3a des-arg were equally reactive in mediating platelet aggregation and release of serotonin. Further C3a and C3a des-arg exhibited synergism with ADP of equal significance in both aggregation and the release reaction. The concentrations of C3a required for the platelet-stimulating activity involve relatively small number of molecules per platelet (4,000-10,000 for the synergistic reaction with ADP). These data suggest the possibility of a C3a (C3a des-arg) receptor on human platelets. This premise is strengthened by the demonstration ultrastructurally of C3a on the platelet membrane subsequent to C3a stimulation.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187348/pdf http://jem.rupress.org/content/158/2/603.full.pdf
ISSN	00221295
DOI	10.1084/jem.158.2.603
Journal	The Journal of experimental medicine
Issue Number	2
Volume Number	158
Language	English
Publisher	Rockefeller University Press
Publisher Date	1983-08-01
Access Restriction	Open
Subject Keyword	Journal: The Journal of experimental medicine Biochemistry and Molecular Biology Carboxypeptidase B Complement C3 Blood Platelets Carboxypeptidases Complement C5
Content Type	Text
Resource Type	Article
Subject	Physiology

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