NDLI: Ex Vivo Isolation and Characterization of Cd4+Cd25+ T Cells with Regulatory Properties from Human Blood

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Ex Vivo Isolation and Characterization of Cd4+Cd25+ T Cells with Regulatory Properties from Human Blood

Content Provider	Scilit
Author	Dieckmann, Detlef Plottner, Heidi Berchtold, Susanne Berger, Thomas Schuler, Gerold
Copyright Year	2001
Description	Journal: The Journal of experimental medicine It has been known for years that rodents harbor a unique population of CD4+CD25+ “professional” regulatory/suppressor T cells that is crucial for the prevention of spontaneous autoimmune diseases. Here we demonstrate that CD4+CD25+CD45RO+ T cells (mean 6% of CD4+ T cells) are present in the blood of adult healthy volunteers. In contrast to previous reports, these CD4+CD25+ T cells do not constitute conventional memory cells but rather regulatory cells exhibiting properties identical to their rodent counterparts. Cytotoxic T lymphocyte–associated antigen (CTLA)-4 (CD152), for example, which is essential for the in vivo suppressive activity of CD4+CD25+ T cells, was constitutively expressed, and remained strongly upregulated after stimulation. The cells were nonproliferative to stimulation via their T cell receptor for antigen, but the anergic state was partially reversed by interleukin (IL)-2 and IL-15. Upon stimulation with allogeneic (but not syngeneic) mature dendritic cells or platebound anti-CD3 plus anti-CD28 the CD4+CD25+ T cells released IL-10, and in coculture experiments suppressed the activation and proliferation of CD4+ and CD8+ T cells. Suppression proved IL-10 independent, yet contact dependent as in the mouse. The identification of regulatory CD4+CD25+ T cells has important implications for the study of tolerance in man, notably in the context of autoimmunity, transplantation, and cancer.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193384/pdf http://jem.rupress.org/content/jem/193/11/1303.full.pdf
ISSN	00221295
DOI	10.1084/jem.193.11.1303
Journal	The Journal of experimental medicine
Issue Number	11
Volume Number	193
Language	English
Publisher	Rockefeller University Press
Publisher Date	2001-06-04
Access Restriction	Open
Subject Keyword	Journal: The Journal of experimental medicine Cd4+cd25+ T Cells Regulatory T Cells Immune Tolerance Dendritic Cells
Content Type	Text
Resource Type	Article
Subject	Physiology

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