NDLI: Augmin accumulation on long-lived microtubules drives amplification and kinetochore-directed growth

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Augmin accumulation on long-lived microtubules drives amplification and kinetochore-directed growth

Content Provider	Scilit
Author	David, Ana F. Roudot, Philippe Legant, Wesley R. Betzig, Eric Danuser, Gaudenz Gerlich, Daniel W.
Copyright Year	2019
Description	Dividing cells reorganize their microtubule cytoskeleton into a bipolar spindle, which moves one set of sister chromatids to each nascent daughter cell. Early spindle assembly models postulated that spindle pole–derived microtubules search the cytoplasmic space until they randomly encounter a kinetochore to form a stable attachment. More recent work uncovered several additional, centrosome-independent microtubule generation pathways, but the contributions of each pathway to spindle assembly have remained unclear. Here, we combined live microscopy and mathematical modeling to show that most microtubules nucleate at noncentrosomal regions in dividing human cells. Using a live-cell probe that selectively labels aged microtubule lattices, we demonstrate that the distribution of growing microtubule plus ends can be almost entirely explained by Augmin-dependent amplification of long-lived microtubule lattices. By ultrafast 3D lattice light-sheet microscopy, we observed that this mechanism results in a strong directional bias of microtubule growth toward individual kinetochores. Our systematic quantification of spindle dynamics reveals highly coordinated microtubule growth during kinetochore fiber assembly.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6605806/pdf https://rupress.org/jcb/article-pdf/218/7/2150/843663/jcb_201805044.pdf
Ending Page	2168
Page Count	19
Starting Page	2150
DOI	10.1083/jcb.201805044
Journal	Journal of Cell Biology
Issue Number	7
Volume Number	218
Language	English
Publisher	Rockefeller University Press
Publisher Date	2019-07-01
Access Restriction	Open
Subject Keyword	Cell Biology Models Lattices Spindle Amplification Kinetochore Augmin Long Lived Microtubules Journal: Journal of Cell Biology (Vol- 218, Issue- 7)
Content Type	Text

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