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Antigen-specific T-cell-mediated suppression. I. Induction of L-glutamic acid(60)-L-alanine(30)-L-tyrosine(10) specific suppressor T cells in vitro requires both antigen-specific T-cell-suppressor factor and antigen
| Content Provider | Scilit |
|---|---|
| Author | Germain, Ronald N. Theze, J. Kapp, J. A. Benacerraf, B. |
| Copyright Year | 1978 |
| Description | Journal: The Journal of experimental medicine A combination of in vitro and in vivo techniques were used to explore the mode of action of both crude and purified suppressive extracts specific for the random copolymer L-giutamic acid(60)-L-alanine(30)-L-tyrosine(10) (GAT- T(s)F) obtained from nonresponder DBA/1 (H-2(q)) mice. Normal DBA/1 spleen cells were incubated under modified Mishell-Dutton culture conditions for 2 days together with crude or purified GAT-T(s)F, and in the presence or absence of free GAT. These cells were then washed extensively and 3 × 10(6) viable cells transferred to syngeneic recipients, which were challenged at the same time with the immunogenic form of GAT complexed to methylated bovine serum albumin (GAT-MBSA). GAT-specific IgG plaque-forming cells (PFC) in the spleen were assayed 7 days later. In agreement with earlier in vitro studies on the action of GAT-T(s)F, it was demonstrated that under these conditions, low concentrations of GAT-T(s)F stimulated the development of cells which, aider transfer, are able to suppress the GAT PFC response to GAT-MBSA. The cells responsible for this suppression were shown to be T lymphocytes by using nylon wool-purified T cells for suppressor cell induction and by eliminating suppressive activity in cells cultured with crude GAT-T(s)F by treatment with anti-Thy 1.2 plus C before transfer. The suppressor T cells act in a specific manner failing to suppress significantly either anti-sheep erythrocyte or trinitrophenyl-ovalbumin primary PFC responses. For the induction of GAT-specific suppressor T cells in culture, a moiety bearing H- 2(K(q) or I(q)) determinants and also GAT, either bound to the crude GAT- T(s)F or added in nanogram amounts to antigen (GAT)-free purified GAT-T(s)F, were both required. |
| Related Links | http://jem.rupress.org/content/147/1/123.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2184087/pdf |
| ISSN | 00221295 |
| DOI | 10.1084/jem.147.1.123 |
| Journal | The Journal of experimental medicine |
| Issue Number | 1 |
| Volume Number | 147 |
| Language | English |
| Publisher | Rockefeller University Press |
| Publisher Date | 1978-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: The Journal of experimental medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology |
