NDLI: Tks5-dependent formation of circumferential podosomes/invadopodia mediates cell–cell fusion

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Tks5-dependent formation of circumferential podosomes/invadopodia mediates cell–cell fusion

Content Provider	Scilit
Author	Oikawa, Tsukasa Oyama, Masaaki Kozuka-Hata, Hiroko Uehara, Shunsuke Udagawa, Nobuyuki Saya, Hideyuki Matsuo, Koichi
Copyright Year	2012
Description	Osteoclasts fuse to form multinucleated cells during osteoclastogenesis. This process is mediated by dynamic rearrangement of the plasma membrane and cytoskeleton, and it requires numerous factors, many of which have been identified. The underlying mechanism remains obscure, however. In this paper, we show that Tks5, a master regulator of invadopodia in cancer cells, is crucial for osteoclast fusion downstream of phosphoinositide 3-kinase and Src. Expression of Tks5 was induced during osteoclastogenesis, and prevention of this induction impaired both the formation of circumferential podosomes and osteoclast fusion without affecting cell differentiation. Tyrosine phosphorylation of Tks5 was attenuated in Src−/− osteoclasts, likely accounting for defects in podosome organization and multinucleation in these cells. Circumferential invadopodia formation in B16F0 melanoma cells was also accompanied by Tks5 phosphorylation. Co-culture of B16F0 cells with osteoclasts in an inflammatory milieu promoted the formation of melanoma–osteoclast hybrid cells. Our results thus reveal an unexpected link between circumferential podosome/invadopodium formation and cell–cell fusion in and beyond osteoclasts.
Related Links	https://rupress.org/jcb/article-pdf/197/4/553/703312/jcb_201111116.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352951/pdf
Ending Page	568
Page Count	16
Starting Page	553
DOI	10.1083/jcb.201111116
Journal	Journal of Cell Biology
Issue Number	4
Volume Number	197
Language	English
Publisher	Rockefeller University Press
Publisher Date	2012-05-14
Access Restriction	Open
Subject Keyword	Cell Biology Phosphorylation Tks5 Circumferential Osteoclasts Osteoclast Fusion Melanoma Podosome B16f0 Mediated Journal: Journal of Cell Biology (Vol- 197, Issue- 4)
Content Type	Text

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