NDLI: ISOLATION OF COATED VESICLES, PLAIN SYNAPTIC VESICLES, AND FLOCCULENT MATERIAL FROM A CRUDE SYNAPTOSOME FRACTION OF GUINEA PIG WHOLE BRAIN

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ISOLATION OF COATED VESICLES, PLAIN SYNAPTIC VESICLES, AND FLOCCULENT MATERIAL FROM A CRUDE SYNAPTOSOME FRACTION OF GUINEA PIG WHOLE BRAIN

Content Provider	Scilit
Author	Kadota, Ken Kadota, Tomoko
Copyright Year	1973
Description	Journal: Journal of Cell Biology Two vesicular fractions and one nonvesicular fraction were prepared from crude synaptosomes by differential centrifugation and salting out with ammonium sulfate. Fraction 1 contained a mixture of coated vesicles, material thought to be derived from breakdown of the coats (shell fragments), and plain synaptic vesicles. Fraction 2 contained a mixture of plain synaptic vesicles and flocculent material. Fraction 3 contained flocculent material only. Fractions 1 and 3 were partially purified by passage through a Sephadex column. Fraction 3 contained no shell fragments but contained finer flocculent material which, it is suggested, is composed of unit particles either occurring singly or linked together into chainlike or amorphous aggregates. Each unit particle appears to have four subunits and is here referred to as a tetrasome. Tetrasomes sometimes appear to be attached to the surfaces of the plain synaptic vesicles. Also, it is possible that aggregates of tetrasomes form part of the structure of the presynaptic dense projections.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109027/pdf http://jcb.rupress.org/content/58/1/135.full.pdf
ISSN	00221295
DOI	10.1083/jcb.58.1.135
Journal	Journal of Cell Biology
Issue Number	1
Volume Number	58
Language	English
Publisher	Rockefeller University Press
Publisher Date	1973-07-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Cell Biology Peripheral Vascular Disease Differential Flocculent Material Plain Synaptic Vesicles
Content Type	Text
Resource Type	Article
Subject	Physiology

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