NDLI: An antibody against secretogranin I (chromogranin B) is packaged into secretory granules.

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An antibody against secretogranin I (chromogranin B) is packaged into secretory granules.

Content Provider	Scilit
Author	Rosa, Patrizia Weiss, U. Pepperkok, Rainer Ansorge, W. Niehrs, C. Stelzer, Ernst H. K. Huttner, W. B.
Copyright Year	1989
Description	We have investigated the sorting and packaging of secretory proteins into secretory granules by an immunological approach. An mAb against secretogranin I (chromogranin B), a secretory protein costored with various peptide hormones and neuropeptides in secretory granules of many endocrine cells and neurons, was expressed by microinjection of its mRNA into the secretogranin I-producing cell line PC12. An mAb against the G protein of vesicular stomatitis virus--i.e., against an antigen not present in PC12 cells--was expressed as a control. The intracellular localization and the secretion of the antibodies was studied by double-labeling immunofluorescence using the conventional and the confocal microscope, as well as by pulse-chase experiments. The secretogranin I antibody, like the control antibody, was transported along the secretory pathway to the Golgi complex. However, in contrast to the control antibody, which was secreted via the constitutive pathway, the secretogranin I antibody formed an immunocomplex with secretogranin I, was packaged into secretory granules, and was released by regulated exocytosis. Our results show that a constitutive secretory protein, unaltered by genetic engineering, can be diverted to the regulated pathway of secretion by its protein-protein interaction with a regulated secretory protein. The data also provide the basis for immunologically studying the role of luminally exposed protein domains in the biogenesis and function of regulated secretory vesicles.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115475/pdf http://jcb.rupress.org/content/109/1/17.full.pdf
Ending Page	34
Page Count	18
Starting Page	17
DOI	10.1083/jcb.109.1.17
Journal	Journal of Cell Biology
Issue Number	1
Volume Number	109
Language	English
Publisher	Rockefeller University Press
Publisher Date	1989-07-01
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Secretory Granules Secretory Protein Packaged Into Secretory Secretogranin I Antibody Journal: Journal of Cell Biology (Vol- 109, Issue- 1)
Content Type	Text

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