NDLI: Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation

Please wait, while we are loading the content...

Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation

Content Provider	Scilit
Author	Wang, Yanming Li, Ming Stadler, Sonja C. Correll, Sarah Li, Pingxin Wang, Danchen Hayama, Ryo Leonelli, Lauriebeth Han, Hyunsil Grigoryev, Sergei A. Allis, C. David Coonrod, Scott A.
Copyright Year	2009
Description	Peripheral blood neutrophils form highly decondensed chromatin structures, termed neutrophil extracellular traps (NETs), that have been implicated in innate immune response to bacterial infection. Neutrophils express high levels of peptidylarginine deiminase 4 (PAD4), which catalyzes histone citrullination. However, whether PAD4 or histone citrullination plays a role in chromatin structure in neutrophils is unclear. In this study, we show that the hypercitrullination of histones by PAD4 mediates chromatin decondensation. Histone hypercitrullination is detected on highly decondensed chromatin in HL-60 granulocytes and blood neutrophils. The inhibition of PAD4 decreases histone hypercitrullination and the formation of NET-like structures, whereas PAD4 treatment of HL-60 cells facilitates these processes. The loss of heterochromatin and multilobular nuclear structures is detected in HL-60 granulocytes after PAD4 activation. Importantly, citrullination of biochemically defined avian nucleosome arrays inhibits their compaction by the linker histone H5 to form higher order chromatin structures. Together, these results suggest that histone hypercitrullination has important functions in chromatin decondensation in granulocytes/neutrophils.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654299/pdf http://jcb.rupress.org/content/184/2/205.full.pdf
Ending Page	213
Page Count	9
Starting Page	205
DOI	10.1083/jcb.200806072
Journal	Journal of Cell Biology
Issue Number	2
Volume Number	184
Language	English
Publisher	Rockefeller University Press
Publisher Date	2009-01-19
Access Restriction	Open
Subject Keyword	Immunology Research and Experimental Medicine Traps Neutrophils Structures Innate Immune Chromatin Decondensation Histone Hypercitrullination Journal: Journal of Cell Biology (Vol- 184, Issue- 2)
Content Type	Text

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in