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Chemotaxis of 3T3 and SV3T3 cells to fibronectin is mediated through the cell-attachment site in fibronectin and a fibronectin cell surface receptor.
| Content Provider | Scilit |
|---|---|
| Author | Albini, Adriana Allavena, G. Melchiori, A. Giancotti, F. Richter, H. Comoglio, Paolo Parodi, S. Martin, G. R. Tarone, Guido |
| Copyright Year | 1987 |
| Description | Fibronectin (FN) is a multidomain extracellular matrix protein that induces attachment and chemotactic migration of fibroblastic cells. In this study we analyzed the molecular determinants involved in the FN-induced chemotactic migration of normal and SV40-transformed 3T3 cells. Two different monoclonal antibodies to the cell-binding site of FN blocked chemotaxis to a 140-kD FN fragment (Ca 140) containing the cell-binding domain. A monoclonal antibody to a determinant distant from the cell-binding site did not affect chemotaxis. A synthetic tetrapeptide, RGDS, which represents the major cell-attachment sequence, was able to compete with FN and the Ca 140 fragment in chemotaxis assays, but this peptide itself had no significant chemotactic activity. A larger peptide encompassing this sequence, GRGDSP, was chemotactic, while the peptide GRGESP, where a glutamic acid residue was substituted for aspartic acid, was inactive. Chemotactic migration could be prevented in a dose-dependent manner by a rabbit polyclonal antiserum to a 140-kD cell surface FN receptor. This antibody was more effective on normal than on transformed 3T3 cells. Neither the anti-FN receptor antiserum nor a monoclonal antibody to the cell-binding site of FN blocked migration induced by another potent chemoattractant, platelet-derived growth factor. These data indicate that FN-induced chemotaxis of 3T3 and SV3T3 cells is mediated via the RGDS cell-attachment site of FN and the 140-kD cell surface FN receptor. The interaction is specific and can be altered by transformation. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114639/pdf http://jcb.rupress.org/content/105/4/1867.full.pdf |
| Ending Page | 1872 |
| Page Count | 6 |
| Starting Page | 1867 |
| DOI | 10.1083/jcb.105.4.1867 |
| Journal | Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 105 |
| Language | English |
| Publisher | Rockefeller University Press |
| Publisher Date | 1987-10-01 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Binding Sites Oligopeptides Chemotaxis Cell Line Journal: Journal of Cell Biology (Vol- 105, Issue- 4) |
| Content Type | Text |
