NDLI: Nucleolar Localization of Human Methionyl–Trna Synthetase and Its Role in Ribosomal RNA Synthesis

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Nucleolar Localization of Human Methionyl–Trna Synthetase and Its Role in Ribosomal RNA Synthesis

Content Provider	Scilit
Author	Ko, Young-Gyu Kang, Young-Sun Kim, Eun-Kyoung Park, Sang Gyu Kim, Sunghoon
Copyright Year	2000
Description	Human aminoacyl–tRNA synthetases (ARSs) are normally located in cytoplasm and are involved in protein synthesis. In the present work, we found that human methionyl–tRNA synthetase (MRS) was translocated to nucleolus in proliferative cells, but disappeared in quiescent cells. The nucleolar localization of MRS was triggered by various growth factors such as insulin, PDGF, and EGF. The presence of MRS in nucleoli depended on the integrity of RNA and the activity of RNA polymerase I in the nucleolus. The ribosomal RNA synthesis was specifically decreased by the treatment of anti-MRS antibody as determined by nuclear run-on assay and immunostaining with anti-Br antibody after incorporating Br-UTP into nascent RNA. Thus, human MRS plays a role in the biogenesis of rRNA in nucleoli, while it is catalytically involved in protein synthesis in cytoplasm.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174846/pdf http://jcb.rupress.org/content/149/3/567.full.pdf
Ending Page	574
Page Count	8
Starting Page	567
DOI	10.1083/jcb.149.3.567
Journal	Journal of Cell Biology
Issue Number	3
Volume Number	149
Language	English
Publisher	Rockefeller University Press
Publisher Date	2000-05-01
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Methionyl–trna Synthetase Nucleoli Growth Signal Ribosomal Rna Synthesis Rna Polymerase I Journal: Journal of Cell Biology (Vol- 149, Issue- 3)
Content Type	Text

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