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Regulated docking of nuclear membrane vesicles to vimentin filaments during mitosis.
| Content Provider | Scilit |
|---|---|
| Author | Maison, C. Horstmann, H. Georgatos, Spyros |
| Copyright Year | 1993 |
| Description | During mitosis, several types of intermediate-sized filaments (IFs) undergo an extensive remodelling in response to phosphorylation by cdc 2 and other protein kinases. However, unlike the nuclear lamins, the cytoplasmic IFs do not seem to follow a fixed disassembly stereotype and often retain their physical continuity without depolymerizing into soluble subunits. To investigate potential interactions between mitotically modified IFs and other cellular structures, we have examined prometaphase-arrested cells expressing the IF protein vimentin. We demonstrate here that vimentin filaments associate in situ and co-fractionate with a distinct population of mitotic vesicles. These vesicles carry on their surfaces nuclear lamin B, the inner nuclear membrane protein p58, and wheat germ agglutinin (WGA)-binding proteins. Consistent with a tight interaction between the IFs and the mitotic membranes, vimentin, nuclear lamin B, and a 180-kD WGA-binding protein are co-isolated when whole mitotic homogenates are incubated with anti-vimentin or anti-lamin B antibodies immobilized on magnetic beads. The vimentin-associated vesicles are essentially depleted of ER, Golgi and endosomal membrane proteins. The interaction of vimentin with lamin B-carrying membranes depends on phosphorylation and is weakened by dephosphorylation during nuclear reassembly in vitro. These observations reveal a novel interaction between IFs and cellular membranes and further suggest that the vimentin filaments may serve as a transient docking site for inner nuclear membrane vesicles during mitosis. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290903/pdf |
| Ending Page | 1505 |
| Page Count | 15 |
| Starting Page | 1491 |
| DOI | 10.1083/jcb.123.6.1491 |
| Journal | Journal of Cell Biology |
| Issue Number | 6 |
| Volume Number | 123 |
| Language | English |
| Publisher | Rockefeller University Press |
| Publisher Date | 1993-12-15 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Developmental Biology Protein Antibodies Phosphorylation Mitosis Vesicles Vimentin Structures Ifs Nuclear Lamin Journal: Journal of Cell Biology (Vol- 123, Issue- 6) |
| Content Type | Text |
