NDLI: Macrophages retain hematopoietic stem cells in the spleen via VCAM-1

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Macrophages retain hematopoietic stem cells in the spleen via VCAM-1

Content Provider	Scilit
Author	Dutta, Partha Hoyer, Friedrich Felix Grigoryeva, Lubov S. Sager, Hendrik B. Leuschner, Florian Courties, Gabriel Borodovsky, Anna Novobrantseva, Tatiana I. Ruda, Vera M. Fitzgerald, Kevin Iwamoto, Yoshiko Wojtkiewicz, Gregory Sun, Yuan Silva, Nicolas Da Libby, Peter Anderson, Daniel Griffith Swirski, Filip Weissleder, Ralph Nahrendorf, Matthias
Copyright Year	2015
Description	Splenic myelopoiesis provides a steady flow of leukocytes to inflamed tissues, and leukocytosis correlates with cardiovascular mortality. Yet regulation of hematopoietic stem cell (HSC) activity in the spleen is incompletely understood. Here, we show that red pulp vascular cell adhesion molecule 1 (VCAM-1)+ macrophages are essential to extramedullary myelopoiesis because these macrophages use the adhesion molecule VCAM-1 to retain HSCs in the spleen. Nanoparticle-enabled in vivo RNAi silencing of the receptor for macrophage colony stimulation factor (M-CSFR) blocked splenic macrophage maturation, reduced splenic VCAM-1 expression and compromised splenic HSC retention. Both, depleting macrophages in CD169 iDTR mice or silencing VCAM-1 in macrophages released HSCs from the spleen. When we silenced either VCAM-1 or M-CSFR in mice with myocardial infarction or in ApoE−/− mice with atherosclerosis, nanoparticle-enabled in vivo RNAi mitigated blood leukocytosis, limited inflammation in the ischemic heart, and reduced myeloid cell numbers in atherosclerotic plaques.
Related Links	https://core.ac.uk/download/pdf/78063635.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387283/pdf http://jem.rupress.org/content/212/4/497.full.pdf
Ending Page	512
Page Count	16
Starting Page	497
DOI	10.1084/jem.20141642
Journal	The Journal of experimental medicine
Issue Number	4
Volume Number	212
Language	English
Publisher	Rockefeller University Press
Publisher Date	2015-04-06
Access Restriction	Open
Subject Keyword	Research and Experimental Medicine Hematopoietic Rnai Macrophages Adhesion Stem Spleen Vcam Leukocytosis Silencing Splenic Journal: The Journal of experimental medicine (Vol- 212, Issue- 4)
Content Type	Text

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